Regulation of pancreatic β-cell insulin secretion by actin cytoskeleton remodelling: Role of gelsolin and cooperation with the MAPK signalling pathway

Alejandra Tomas, Barbara Yermen, Le Min, Jeffrey E. Pessin, Philippe A. Halban

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

We have previously isolated two MIN6 β-cell sublines, B1, highly responsive to glucose-stimulated insulin secretion, and C3, markedly refractory (Lilla, V., Webb, G., Rickenbach, K., Maturana, A., Steiner, D. F., Halban, P. A. and Irminger, J. C. (2003) Endocrinology 144, 1368-1379). We now demonstrate that C3 cells have substantially increased amounts of F-actin stress fibres whereas B1 cells have shorter cortical F-actin. Consistent with these data, B1 cells display glucose-dependent actin remodelling whereas, in C3 cells, F-actin is refractory to this secretagogue. Furthermore, F-actin depolymerisation with latrunculin B restores glucose-stimulated insulin secretion in C3 cells. In parallel, glucose-stimulated ERK1/2 activation is greater in B1 than in C3 cells, and is potentiated in both sublines following F-actin depolymerisation. Glucose-activated phosphoERK1/2 accumulates at actin filament tips adjacent to the plasma membrane, indicating that these are the main sites of action for this kinase during insulin secretion. In addition, B1 cell expression of the calcium-dependent F-actin severing protein gelsolin is >100-fold higher than that of C3 cells. Knock-down of gelsolin reduced glucose-stimulated insulin secretion, whereas gelsolin over-expression potentiated secretion from B1 cells. Gelsolin localised along depolymerised actin fibres after glucose stimulation. Taken together, these data demonstrate that F-actin reorganization prior to insulin secretion requires gelsolin and plays a role in the glucose-dependent MAPK signal transduction that regulates β-cell insulin secretion.

Original languageEnglish (US)
Pages (from-to)2156-2167
Number of pages12
JournalJournal of Cell Science
Volume119
Issue number10
DOIs
StatePublished - May 15 2006
Externally publishedYes

Fingerprint

Gelsolin
Actin Cytoskeleton
Actins
Insulin
Glucose
Stress Fibers
Endocrinology
Signal Transduction
Phosphotransferases

Keywords

  • Actin dynamics
  • Beta cells
  • ERK1/2
  • Gelsolin
  • Insulin secretion
  • Latrunculin B

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of pancreatic β-cell insulin secretion by actin cytoskeleton remodelling : Role of gelsolin and cooperation with the MAPK signalling pathway. / Tomas, Alejandra; Yermen, Barbara; Min, Le; Pessin, Jeffrey E.; Halban, Philippe A.

In: Journal of Cell Science, Vol. 119, No. 10, 15.05.2006, p. 2156-2167.

Research output: Contribution to journalArticle

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AU - Yermen, Barbara

AU - Min, Le

AU - Pessin, Jeffrey E.

AU - Halban, Philippe A.

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