Regulation of human Gc (vitamin d-binding) protein levels

Hormonal and cytokine control of gene expression in vitro

Chandan Guha, Motoki Osawa, Phillip A. Werner, Robert M. Galbraith, Gary V. Paddock

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Studies were performed in Hep3B hepatocytes to better elucidate the mechanisms regulating circulating levels of human group-specific component (Gc). We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting from treatment of hepatocytes with cytokines and hormones known to influence synthesis of other proteins of hepatic origin. We particularly focused on compounds known to be prototypic stimulants during the acute phase response. Interleukin-6 (IL-6) and dexamethasone were shown to increase Gc mRNA approximately twofold while transforming growth factor beta (TGFβ) decreased Gc mRNA in a dose-dependent fashion by up to fivefold. The effects on secreted Gc protein levels were similar. These results indicate that Gc protein appears to be regulated differently than the other members of this gene family, albumin and alpha-fetoprotein (AFP), which are negative acute phase reactants. In addition, these contrasting effects on Gc synthesis of IL-6 and dexamethasone and of TGFβ suggest that high basal levels of Gc synthesis may be maintained during the acute phase response.

Original languageEnglish (US)
Pages (from-to)1675-1681
Number of pages7
JournalHepatology
Volume21
Issue number6
DOIs
StatePublished - 1995
Externally publishedYes

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Vitamins
Carrier Proteins
Cytokines
Gene Expression
Acute-Phase Reaction
Transforming Growth Factor beta
Messenger RNA
Dexamethasone
Hepatocytes
Interleukin-6
Proteins
Acute-Phase Proteins
alpha-Fetoproteins
Albumins
Hormones
In Vitro Techniques
Liver
Genes

ASJC Scopus subject areas

  • Hepatology

Cite this

Regulation of human Gc (vitamin d-binding) protein levels : Hormonal and cytokine control of gene expression in vitro. / Guha, Chandan; Osawa, Motoki; Werner, Phillip A.; Galbraith, Robert M.; Paddock, Gary V.

In: Hepatology, Vol. 21, No. 6, 1995, p. 1675-1681.

Research output: Contribution to journalArticle

Guha, Chandan ; Osawa, Motoki ; Werner, Phillip A. ; Galbraith, Robert M. ; Paddock, Gary V. / Regulation of human Gc (vitamin d-binding) protein levels : Hormonal and cytokine control of gene expression in vitro. In: Hepatology. 1995 ; Vol. 21, No. 6. pp. 1675-1681.
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