Regulation of glucose transporter messenger RNA levels in rat adipose tissue by insulin

William I. Sivitz, Susan L. DeSautel, Toshiaki Kayano, Graeme I. Bell, Jeffrey E. Pessin

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Analysis of glucose transporter mRNA levels in adipose tissue from streptozotocin (STZ)-induced diabetic rats demonstrated a specific decrease (10-fold) in adipose tissue GLUT-4 mRNA with no significant effect on GLUT-1 mRNA levels. Treatment of STZ-diabetic rats with twice daily injections of insulin for 1-3 days resulted in a 16-fold increase in the relative amount of GLUT-4 mRNA to levels approximately 2-fold greater than those in control animals. However, after 7 days of insulin therapy the amount of GLUT-4 mRNA decreased approximately 2-fold back to the levels in the control animals. Normalization of the STZ-induced serum hyperglycemia by phlorizin treatment, which inhibits renal tubular reabsorption of glucose, had no effect on GLUT-4 mRNA in the absence of insulin. Similar to STZ-diabetes, fasting for 48 h also reduced adipose GLUT-4 mRNA levels. Parenteral administration of insulin with glucose over 7.5 h, but not glucose alone, increased the levels of the GLUT-4 mRNA 3-to 4-fold. These studies demonstrate that the relative glycemic state does not influence GLUT-4 glucose transporter mRNA expression in vivo and strongly suggests that insulin is a major factor regulating the levels of GLUT-4 mRNA in adipose tissue.

Original languageEnglish (US)
Pages (from-to)583-588
Number of pages6
JournalMolecular Endocrinology
Volume4
Issue number4
StatePublished - Apr 1990
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Adipose Tissue
Insulin
Messenger RNA
Streptozocin
Glucose
Phlorhizin
Experimental Diabetes Mellitus
Hyperglycemia
Fasting
Therapeutics
Injections

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Regulation of glucose transporter messenger RNA levels in rat adipose tissue by insulin. / Sivitz, William I.; DeSautel, Susan L.; Kayano, Toshiaki; Bell, Graeme I.; Pessin, Jeffrey E.

In: Molecular Endocrinology, Vol. 4, No. 4, 04.1990, p. 583-588.

Research output: Contribution to journalArticle

Sivitz, WI, DeSautel, SL, Kayano, T, Bell, GI & Pessin, JE 1990, 'Regulation of glucose transporter messenger RNA levels in rat adipose tissue by insulin', Molecular Endocrinology, vol. 4, no. 4, pp. 583-588.
Sivitz, William I. ; DeSautel, Susan L. ; Kayano, Toshiaki ; Bell, Graeme I. ; Pessin, Jeffrey E. / Regulation of glucose transporter messenger RNA levels in rat adipose tissue by insulin. In: Molecular Endocrinology. 1990 ; Vol. 4, No. 4. pp. 583-588.
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