Regression mapping of association between the human leukocyte antigen region and Graves disease

Matthew J. Simmonds, Joanna M M Howson, Joanne M. Heward, Heather J. Cordell, Helen Foxall, Jackie Carr-Smith, Sarah M. Gibson, Neil Walker, Yaron Tomer, Jayne A. Franklyn, John A. Todd, Stephen C L Gough

Research output: Contribution to journalArticle

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Abstract

The human leukocyte antigen class II genes DRB1, DQB1, and DQA1 are associated with Graves disease (GD), but, because of strong linkage disequilibrium within this region, the primary etiological variant(s) remains unknown. In the present study, 871 patients with GD and 621 control subjects were genotyped at the DRB1, DQB1, and DQA1 loci. All three loci were associated with GD (P = 1.45 × 10-12, P = 3.20 × 10-5, and P = 9.26 × 10-12, respectively). Stepwise logistic-regression analysis showed that the association could be explained by either DRB1 or DQA1 but not by DQB1. To extend previous results, the amino acid sequence of the exon 2-encoded peptide-binding domain of DRB1 was predicted for each subject, and, by use of logistic regression, each position was analyzed for association with GD. Of 102 amino acids, 70 were uninformative; of the remaining 32 amino acids, 13 were associated with GD (P values ranged from 2.20 × 10-4 to 1.2 × 10-12). The strongest association was at position β74. This analysis is consistent with the possibility that position β74 of exon 2 of the DRB1 molecule may have a specific and central role in autoantigen presentation by DRB1 to T lymphocytes. However, we cannot yet exclude a primary role for DQA1 or for other polymorphisms that affect DRB1 function or expression.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalAmerican Journal of Human Genetics
Volume76
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

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Graves Disease
HLA Antigens
Exons
Logistic Models
Amino Acids
MHC Class II Genes
Linkage Disequilibrium
Autoantigens
Amino Acid Sequence
Regression Analysis
T-Lymphocytes

ASJC Scopus subject areas

  • Genetics

Cite this

Simmonds, M. J., Howson, J. M. M., Heward, J. M., Cordell, H. J., Foxall, H., Carr-Smith, J., ... Gough, S. C. L. (2005). Regression mapping of association between the human leukocyte antigen region and Graves disease. American Journal of Human Genetics, 76(1), 157-163. https://doi.org/10.1086/426947

Regression mapping of association between the human leukocyte antigen region and Graves disease. / Simmonds, Matthew J.; Howson, Joanna M M; Heward, Joanne M.; Cordell, Heather J.; Foxall, Helen; Carr-Smith, Jackie; Gibson, Sarah M.; Walker, Neil; Tomer, Yaron; Franklyn, Jayne A.; Todd, John A.; Gough, Stephen C L.

In: American Journal of Human Genetics, Vol. 76, No. 1, 01.2005, p. 157-163.

Research output: Contribution to journalArticle

Simmonds, MJ, Howson, JMM, Heward, JM, Cordell, HJ, Foxall, H, Carr-Smith, J, Gibson, SM, Walker, N, Tomer, Y, Franklyn, JA, Todd, JA & Gough, SCL 2005, 'Regression mapping of association between the human leukocyte antigen region and Graves disease', American Journal of Human Genetics, vol. 76, no. 1, pp. 157-163. https://doi.org/10.1086/426947
Simmonds MJ, Howson JMM, Heward JM, Cordell HJ, Foxall H, Carr-Smith J et al. Regression mapping of association between the human leukocyte antigen region and Graves disease. American Journal of Human Genetics. 2005 Jan;76(1):157-163. https://doi.org/10.1086/426947
Simmonds, Matthew J. ; Howson, Joanna M M ; Heward, Joanne M. ; Cordell, Heather J. ; Foxall, Helen ; Carr-Smith, Jackie ; Gibson, Sarah M. ; Walker, Neil ; Tomer, Yaron ; Franklyn, Jayne A. ; Todd, John A. ; Gough, Stephen C L. / Regression mapping of association between the human leukocyte antigen region and Graves disease. In: American Journal of Human Genetics. 2005 ; Vol. 76, No. 1. pp. 157-163.
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