Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria

Richard P. Kennan, Fabiana S. Machado, Sunhee C. Lee, Mahalia S. Desruisseaux, Murray Wittner, Moriya Tsuji, Herbert B. Tanowitz

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Cerebral malaria is an important cause of morbidity and mortality in many parts of the world. It has been suggested that cerebral malaria is associated with reduced perfusion due to the blockage of blood vessels by parasitized erythrocytes; although, no quantitative validation of this has been done. We infected C57BL/6 mice with the ANKA strain of Plasmodium berghei and on day 6 of infection we investigated alterations in brain function using arterial spin labeling MRI and proton MRS. MR images did not demonstrate signs of damage. However, there was a significant reduction in cerebral blood flow (P<0.012) and the ratio of N-acetyl-aspartate (NAA) to creatine (Cr) (P<0.01) relative to non-infected mice. The NAA/Cr ratios were significantly correlated with cerebral perfusion (r=0.87) suggesting a relationship between impaired oxygen delivery and neuronal dysfunction. Pathological examination revealed accumulations of damaged axons providing a correlate for the decreased NAA/Cr ratio in infected mice. This murine model will permit non-invasive studies of neurologic function during malarial infection.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalParasitology Research
Volume96
Issue number5
DOIs
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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    Kennan, R. P., Machado, F. S., Lee, S. C., Desruisseaux, M. S., Wittner, M., Tsuji, M., & Tanowitz, H. B. (2005). Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria. Parasitology Research, 96(5), 302-307. https://doi.org/10.1007/s00436-005-1349-z