Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria

Richard P. Kennan, Fabiana S. Machado, Sunhee C. Lee, Mahalia S. Desruisseaux, Murray Wittner, Moriya Tsuji, Herbert B. Tanowitz

Research output: Contribution to journalArticle

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Abstract

Cerebral malaria is an important cause of morbidity and mortality in many parts of the world. It has been suggested that cerebral malaria is associated with reduced perfusion due to the blockage of blood vessels by parasitized erythrocytes; although, no quantitative validation of this has been done. We infected C57BL/6 mice with the ANKA strain of Plasmodium berghei and on day 6 of infection we investigated alterations in brain function using arterial spin labeling MRI and proton MRS. MR images did not demonstrate signs of damage. However, there was a significant reduction in cerebral blood flow (P<0.012) and the ratio of N-acetyl-aspartate (NAA) to creatine (Cr) (P<0.01) relative to non-infected mice. The NAA/Cr ratios were significantly correlated with cerebral perfusion (r=0.87) suggesting a relationship between impaired oxygen delivery and neuronal dysfunction. Pathological examination revealed accumulations of damaged axons providing a correlate for the decreased NAA/Cr ratio in infected mice. This murine model will permit non-invasive studies of neurologic function during malarial infection.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalParasitology Research
Volume96
Issue number5
DOIs
StatePublished - Jul 2005

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Cerebrovascular Circulation
Cerebral Malaria
creatine
Creatine
aspartic acid
blood flow
animal models
mice
Perfusion
Plasmodium berghei
Infection
Inbred C57BL Mouse
blood vessels
nervous system
axons
infection
Nervous System
protons
Blood Vessels
Axons

ASJC Scopus subject areas

  • Parasitology

Cite this

Kennan, R. P., Machado, F. S., Lee, S. C., Desruisseaux, M. S., Wittner, M., Tsuji, M., & Tanowitz, H. B. (2005). Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria. Parasitology Research, 96(5), 302-307. https://doi.org/10.1007/s00436-005-1349-z

Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria. / Kennan, Richard P.; Machado, Fabiana S.; Lee, Sunhee C.; Desruisseaux, Mahalia S.; Wittner, Murray; Tsuji, Moriya; Tanowitz, Herbert B.

In: Parasitology Research, Vol. 96, No. 5, 07.2005, p. 302-307.

Research output: Contribution to journalArticle

Kennan, RP, Machado, FS, Lee, SC, Desruisseaux, MS, Wittner, M, Tsuji, M & Tanowitz, HB 2005, 'Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria', Parasitology Research, vol. 96, no. 5, pp. 302-307. https://doi.org/10.1007/s00436-005-1349-z
Kennan RP, Machado FS, Lee SC, Desruisseaux MS, Wittner M, Tsuji M et al. Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria. Parasitology Research. 2005 Jul;96(5):302-307. https://doi.org/10.1007/s00436-005-1349-z
Kennan, Richard P. ; Machado, Fabiana S. ; Lee, Sunhee C. ; Desruisseaux, Mahalia S. ; Wittner, Murray ; Tsuji, Moriya ; Tanowitz, Herbert B. / Reduced cerebral blood flow and N-acetyl aspartate in a murine model of cerebral malaria. In: Parasitology Research. 2005 ; Vol. 96, No. 5. pp. 302-307.
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