@article{21e5c29536c14bb8a598add4652e4341,
title = "Recognition of polyadenylate RNA by the poly(A)-binding protein",
abstract = "The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 {\AA} resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type RNA-binding domains connected by a short linker (RRM1/2). These two RRMs form a continuous RNA-binding trough, lined by an antiparallel β sheet backed by four α helices. The polyadenylate RNA adopts an extended conformation running the length of the molecular trough. Adenine recognition is primarily mediated by contacts with conserved residues found in the RNP motifs of the two RRMs. The convex dorsum of RRM1/2 displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with translation initiation factors and regulatory proteins.",
author = "Deo, {Rahul C.} and Bonanno, {Jeffrey B.} and Nahum Sonenberg and Burley, {Stephen K.}",
note = "Funding Information: At the National Synchrotron Light Source, we thank Dr. Zbigniew Dauter for his help using Beamline X9B, and at the Cornell High Energy Synchrotron Source we thank Dr. Dan Thiel and the MacCHESS staff for their help using Beamlines A1 and F1. We thank Dr. S. C. Almo, Dr. G. Blobel, Dr. A. W. Craig, Dr. J. E. Darnell, Dr. A. R. Ferr{\'e}-D'Amar{\'e}, Dr. K. S. Gajiwala, Dr. A.-C. Gingras, Dr. M. Huse, Dr. H. Imataka, Dr. D. Jeruzalmi, Dr. A. Kahwajian, Dr. K. Kamada, Dr. J. Kuriyan, Dr. H. Lewis, Dr. J. Marcotrigiano, Dr. S. K. Nair, Dr. G. A. Petsko, Dr. A. Sali, Dr. S. Scaringe, Dr. S. Soisson, Dr. B. Strokopytov, and Dr. M. Young for many useful discussions. S. K. B. is an Investigator in the Howard Hughes Medical Institute, and N. S. is a Howard Hughes Medical Institute International Scholar. This work was supported in part by the Rockefeller University (S. K. B.) and the Medical Research Council of Canada (N. S.). R. C. D. was supported by the Cornell/Rockefeller/Sloan-Kettering Tri-Institutional MD-PhD Program and the Andrew W. Mellon Foundation.",
year = "1999",
month = sep,
day = "17",
doi = "10.1016/S0092-8674(00)81517-2",
language = "English (US)",
volume = "98",
pages = "835--845",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}