TY - JOUR
T1 - Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes
AU - Krishnakumar, Raga
AU - Gamble, Matthew J.
AU - Frizzell, Kristine M.
AU - Berrocal, Jhoanna G.
AU - Kininis, Miltiadis
AU - Kraus, W. Lee
PY - 2008/2/8
Y1 - 2008/2/8
N2 - Nucleosome-binding proteins act to modulate the promoter chromatin architecture and transcription of target genes. We used genomic and gene-specific approaches to show that two such factors, histone H1 and poly(ADP-ribose) polymerase-1 (PARP-1), exhibit a reciprocal pattern of chromatin binding at many RNA polymerase II-transcribed promoters. PARP-1 was enriched and H1 was depleted at these promoters. This pattern of binding was associated with actively transcribed genes. Furthermore, we showed that PARP-1 acts to exclude H1 from a subset of PARP-1-stimulated promoters, suggesting a functional interplay between PARP-1 and H1 at the level of nucleosome binding. Thus, although H1 and PARP-1 have similar nucleosome-binding properties and effects on chromatin structure in vitro, they have distinct roles in determining gene expression outcomes in vivo.
AB - Nucleosome-binding proteins act to modulate the promoter chromatin architecture and transcription of target genes. We used genomic and gene-specific approaches to show that two such factors, histone H1 and poly(ADP-ribose) polymerase-1 (PARP-1), exhibit a reciprocal pattern of chromatin binding at many RNA polymerase II-transcribed promoters. PARP-1 was enriched and H1 was depleted at these promoters. This pattern of binding was associated with actively transcribed genes. Furthermore, we showed that PARP-1 acts to exclude H1 from a subset of PARP-1-stimulated promoters, suggesting a functional interplay between PARP-1 and H1 at the level of nucleosome binding. Thus, although H1 and PARP-1 have similar nucleosome-binding properties and effects on chromatin structure in vitro, they have distinct roles in determining gene expression outcomes in vivo.
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U2 - 10.1126/science.1149250
DO - 10.1126/science.1149250
M3 - Article
C2 - 18258916
AN - SCOPUS:38949198773
SN - 0036-8075
VL - 319
SP - 819
EP - 821
JO - Science
JF - Science
IS - 5864
ER -