Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes

Raga Krishnakumar, Matthew J. Gamble, Kristine M. Frizzell, Jhoanna G. Berrocal, Miltiadis Kininis, W. Lee Kraus

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

Nucleosome-binding proteins act to modulate the promoter chromatin architecture and transcription of target genes. We used genomic and gene-specific approaches to show that two such factors, histone H1 and poly(ADP-ribose) polymerase-1 (PARP-1), exhibit a reciprocal pattern of chromatin binding at many RNA polymerase II-transcribed promoters. PARP-1 was enriched and H1 was depleted at these promoters. This pattern of binding was associated with actively transcribed genes. Furthermore, we showed that PARP-1 acts to exclude H1 from a subset of PARP-1-stimulated promoters, suggesting a functional interplay between PARP-1 and H1 at the level of nucleosome binding. Thus, although H1 and PARP-1 have similar nucleosome-binding properties and effects on chromatin structure in vitro, they have distinct roles in determining gene expression outcomes in vivo.

Original languageEnglish (US)
Pages (from-to)819-821
Number of pages3
JournalScience
Volume319
Issue number5864
DOIs
StatePublished - Feb 8 2008
Externally publishedYes

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Histones
Nucleosomes
Chromatin
Genes
RNA Polymerase II
Poly (ADP-Ribose) Polymerase-1
Carrier Proteins
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Krishnakumar, R., Gamble, M. J., Frizzell, K. M., Berrocal, J. G., Kininis, M., & Kraus, W. L. (2008). Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. Science, 319(5864), 819-821. https://doi.org/10.1126/science.1149250

Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. / Krishnakumar, Raga; Gamble, Matthew J.; Frizzell, Kristine M.; Berrocal, Jhoanna G.; Kininis, Miltiadis; Kraus, W. Lee.

In: Science, Vol. 319, No. 5864, 08.02.2008, p. 819-821.

Research output: Contribution to journalArticle

Krishnakumar, R, Gamble, MJ, Frizzell, KM, Berrocal, JG, Kininis, M & Kraus, WL 2008, 'Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes', Science, vol. 319, no. 5864, pp. 819-821. https://doi.org/10.1126/science.1149250
Krishnakumar, Raga ; Gamble, Matthew J. ; Frizzell, Kristine M. ; Berrocal, Jhoanna G. ; Kininis, Miltiadis ; Kraus, W. Lee. / Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. In: Science. 2008 ; Vol. 319, No. 5864. pp. 819-821.
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