REB1, a yeast DNA-binding protein with many targets, is essential for cell growth and bears some resemblance to the oncogene myb

J. U. Qida, Bernice E. Morrow, Jonathan R. Warner

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

REB1 is a DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription as well as upstream of many genes transcribed by RNA polymerase II. We report here the cloning of the gene for REB1 by screening a yeast genomic Agt11 library with specific oligonucleotides containing the REB1 binding site consensus sequence. The REB1 gene was sequenced, revealing an open reading frame encoding 809 amino acids. The predicted protein was highly hydrophilic, with numerous OH-containing amino acids and glutamines, features common to many of the general DNA-binding proteins of Saccharomyces cerevisiae, such as ABF1, RAP1, GCN4, and HSF1. There was some homology between a portion of REB1 and the DNA-binding domain of the oncogene myb. REB1 is an essential gene that maps on chromosome II. However, the physiological role that it plays in the cell has yet to be established.

Original languageEnglish (US)
Pages (from-to)5226-5234
Number of pages9
JournalMolecular and Cellular Biology
Volume10
Issue number10
StatePublished - 1990

Fingerprint

myb Genes
Fungal Proteins
DNA-Binding Proteins
Growth
Genes
Amino Acids
Genomic Library
RNA Polymerase II
Essential Genes
Consensus Sequence
Glutamine
Genetic Promoter Regions
Oligonucleotides
Open Reading Frames
Saccharomyces cerevisiae
Organism Cloning
Chromosomes
Yeasts
Binding Sites
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

REB1, a yeast DNA-binding protein with many targets, is essential for cell growth and bears some resemblance to the oncogene myb. / Qida, J. U.; Morrow, Bernice E.; Warner, Jonathan R.

In: Molecular and Cellular Biology, Vol. 10, No. 10, 1990, p. 5226-5234.

Research output: Contribution to journalArticle

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