Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship

Samuel L. Aitken, Vagish S. Hemmige, Hoonmo L. Koo, Nancy N. Vuong, Todd M. Lasco, Kevin W. Garey

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for antimicrobial stewardship programs. A total of 143 consecutive patients with Gram-positive bacteremia were included in the analysis. BC-GP correctly identified 127/128 (99.2%) of organisms from monomicrobial blood cultures and 9/14 (64.3%) from polymicrobial, including all methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. Stewardship interventions were possible in 51.0% of patients, most commonly stopping or preventing unnecessary vancomycin or starting a targeted therapy. In Monte Carlo simulations, unnecessary antibiotics could be stopped at least 24 hours earlier in 65.6% of cases, and targeted therapy could be started at least 24 hours earlier in 81.2%. BC-GP is a potentially useful test for antibiotic stewardship in patients with Gram-positive bacteremia.

Original languageEnglish (US)
Pages (from-to)4-8
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume81
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Bacteremia
  • Enterococcus
  • Prospective cohort
  • Staphylococcus
  • Staphylococcus aureus
  • Streptococcus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship'. Together they form a unique fingerprint.

Cite this