Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship

Samuel L. Aitken, Vagish S. Hemmige, Hoonmo L. Koo, Nancy N. Vuong, Todd M. Lasco, Kevin W. Garey

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for antimicrobial stewardship programs. A total of 143 consecutive patients with Gram-positive bacteremia were included in the analysis. BC-GP correctly identified 127/128 (99.2%) of organisms from monomicrobial blood cultures and 9/14 (64.3%) from polymicrobial, including all methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. Stewardship interventions were possible in 51.0% of patients, most commonly stopping or preventing unnecessary vancomycin or starting a targeted therapy. In Monte Carlo simulations, unnecessary antibiotics could be stopped at least 24 hours earlier in 65.6% of cases, and targeted therapy could be started at least 24 hours earlier in 81.2%. BC-GP is a potentially useful test for antibiotic stewardship in patients with Gram-positive bacteremia.

Original languageEnglish (US)
Pages (from-to)4-8
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume81
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Bacteremia
Infection
Anti-Bacterial Agents
Vancomycin
Methicillin-Resistant Staphylococcus aureus
Routine Diagnostic Tests
Therapeutics
Blood Culture
Vancomycin-Resistant Enterococci

Keywords

  • Bacteremia
  • Enterococcus
  • Prospective cohort
  • Staphylococcus
  • Staphylococcus aureus
  • Streptococcus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship. / Aitken, Samuel L.; Hemmige, Vagish S.; Koo, Hoonmo L.; Vuong, Nancy N.; Lasco, Todd M.; Garey, Kevin W.

In: Diagnostic Microbiology and Infectious Disease, Vol. 81, No. 1, 01.01.2015, p. 4-8.

Research output: Contribution to journalArticle

@article{482c417f08404469870a1ac9f20c8309,
title = "Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship",
abstract = "The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for antimicrobial stewardship programs. A total of 143 consecutive patients with Gram-positive bacteremia were included in the analysis. BC-GP correctly identified 127/128 (99.2{\%}) of organisms from monomicrobial blood cultures and 9/14 (64.3{\%}) from polymicrobial, including all methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. Stewardship interventions were possible in 51.0{\%} of patients, most commonly stopping or preventing unnecessary vancomycin or starting a targeted therapy. In Monte Carlo simulations, unnecessary antibiotics could be stopped at least 24 hours earlier in 65.6{\%} of cases, and targeted therapy could be started at least 24 hours earlier in 81.2{\%}. BC-GP is a potentially useful test for antibiotic stewardship in patients with Gram-positive bacteremia.",
keywords = "Bacteremia, Enterococcus, Prospective cohort, Staphylococcus, Staphylococcus aureus, Streptococcus",
author = "Aitken, {Samuel L.} and Hemmige, {Vagish S.} and Koo, {Hoonmo L.} and Vuong, {Nancy N.} and Lasco, {Todd M.} and Garey, {Kevin W.}",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.diagmicrobio.2014.09.025",
language = "English (US)",
volume = "81",
pages = "4--8",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship

AU - Aitken, Samuel L.

AU - Hemmige, Vagish S.

AU - Koo, Hoonmo L.

AU - Vuong, Nancy N.

AU - Lasco, Todd M.

AU - Garey, Kevin W.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for antimicrobial stewardship programs. A total of 143 consecutive patients with Gram-positive bacteremia were included in the analysis. BC-GP correctly identified 127/128 (99.2%) of organisms from monomicrobial blood cultures and 9/14 (64.3%) from polymicrobial, including all methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. Stewardship interventions were possible in 51.0% of patients, most commonly stopping or preventing unnecessary vancomycin or starting a targeted therapy. In Monte Carlo simulations, unnecessary antibiotics could be stopped at least 24 hours earlier in 65.6% of cases, and targeted therapy could be started at least 24 hours earlier in 81.2%. BC-GP is a potentially useful test for antibiotic stewardship in patients with Gram-positive bacteremia.

AB - The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for antimicrobial stewardship programs. A total of 143 consecutive patients with Gram-positive bacteremia were included in the analysis. BC-GP correctly identified 127/128 (99.2%) of organisms from monomicrobial blood cultures and 9/14 (64.3%) from polymicrobial, including all methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. Stewardship interventions were possible in 51.0% of patients, most commonly stopping or preventing unnecessary vancomycin or starting a targeted therapy. In Monte Carlo simulations, unnecessary antibiotics could be stopped at least 24 hours earlier in 65.6% of cases, and targeted therapy could be started at least 24 hours earlier in 81.2%. BC-GP is a potentially useful test for antibiotic stewardship in patients with Gram-positive bacteremia.

KW - Bacteremia

KW - Enterococcus

KW - Prospective cohort

KW - Staphylococcus

KW - Staphylococcus aureus

KW - Streptococcus

UR - http://www.scopus.com/inward/record.url?scp=84916604425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84916604425&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2014.09.025

DO - 10.1016/j.diagmicrobio.2014.09.025

M3 - Article

VL - 81

SP - 4

EP - 8

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 1

ER -