Reactivity of the amino groups of carbonmonoxyhemoglobin S with glyceraldehyde.

A. S. Acharya, J. M. Manning

Research output: Contribution to journalArticle

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Abstract

The inhibition of erythrocyte sickling in vitro by glyceraldehyde has been shown previously to result from a reduced gelation of deoxyhemoglobin S. Accordingly, the sites of Schiff base formation of the sugar aldehyde with hemoglobin have been determined by peptide of the protein after treatment of CO-saturated sickle cells with 10 mM [14C]glyceraldehyde for 90 min and reduction with NaBH4. About 23% of the glyceraldehyde incorporated into hemoglobin was present at Val-1(beta) with very little present at Val-1(alpha) (less than 5%. The distribution of [14C]glyceraldehyde between the hemoglobin chains was 60% in the beta chains and 40% in the alpha chains. The reactive lysine residues of the beta chain were Lys-82, Lys-59, and Lys-120 (45%, 20%, and 16% of the total 14C in the beta chains, respectively). The most reactive lysine residue of the alpha chain was Lys-16 where 75% of the total [14C]glyceraldehyde was present. The limited number of sites reactive with glyceraldehyde indicate some type of selectivity in the reaction of sugar aldehydes with hemoglobin.

Original languageEnglish (US)
Pages (from-to)1406-1412
Number of pages7
JournalJournal of Biological Chemistry
Volume255
Issue number4
StatePublished - Feb 25 1980
Externally publishedYes

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Glyceraldehyde
Carboxyhemoglobin
Hemoglobins
Aldehydes
Sugars
Lysine
Sickle Hemoglobin
Schiff Bases
Gelation
Carbon Monoxide
Catalytic Domain
Erythrocytes
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Reactivity of the amino groups of carbonmonoxyhemoglobin S with glyceraldehyde. / Acharya, A. S.; Manning, J. M.

In: Journal of Biological Chemistry, Vol. 255, No. 4, 25.02.1980, p. 1406-1412.

Research output: Contribution to journalArticle

Acharya, A. S. ; Manning, J. M. / Reactivity of the amino groups of carbonmonoxyhemoglobin S with glyceraldehyde. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 4. pp. 1406-1412.
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