Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants

Dianne Cox, Deborah Wessels, David R. Soll, John Hartwig, John S. Condeelis

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Abstract

The actin binding protein ABP-120 has been proposed to cross-link actin filaments in nascent pseudopods, in a step required for normal pseudopod extension in motile Dictyostelium amoebae. To test this hypothesis, cell lines that lack ABP-120 were created independently either by chemical mutagenesis or homologous recombination. Different phenotypes were reported in these two studies. The chemical mutant shows only a subtle defect in actin cross-linking, while the homologous recombinant mutants show profound defects in actin cross-linking, cytoskeletal structure, pseudopod number and size, cell motility and chemotaxis and, as shown here, phagocytosis. To resolve the controversy as to what the ABP-120 phenotype is, ABP-120 was re-expressed in an ABP-120 cell line created by homologous recombination. Two independently 'rescued' cell lines that express wild-type levels of ABP-120 were analyzed. In both rescued cell lines, actin incorporation into the cytoskeleton, pseudopod formation, cell morphology, instantaneous velocity, phagocytosis, and chemotaxis were restored to wild-type levels. There is no alteration in the expression levels of several related actin binding proteins in either the original ABP-120- cell line or in the rescued cell lines, leading to the conclusion that neither the aberrant phenotype observed in ABP-120- cells nor the normal phenotype reasserted in rescued cells can be attributed to alterations in the levels of other abundant and related actin binding proteins. Re-expression of ABP-120 in ABP-120- cells reestablishes normal structural and behavioral parameters, demonstrating that the severity and properties of the structural and behavioral defects of ABP-120- cell lines produced by homologous recombination are the direct result of the absence of ABP-120.

Original languageEnglish (US)
Pages (from-to)803-823
Number of pages21
JournalMolecular Biology of the Cell
Volume7
Issue number5
StatePublished - May 1996

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Dictyostelium
Phagocytosis
Pseudopodia
Cell Line
Microfilament Proteins
Homologous Recombination
Phenotype
Actins
Chemotaxis
Amoeba
Cytoskeleton
Actin Cytoskeleton
Mutagenesis
Cell Movement

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants. / Cox, Dianne; Wessels, Deborah; Soll, David R.; Hartwig, John; Condeelis, John S.

In: Molecular Biology of the Cell, Vol. 7, No. 5, 05.1996, p. 803-823.

Research output: Contribution to journalArticle

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