Rate and extent of phagocytosis in macrophages lacking vamp3

Lee Ann H Allen, Chunmei Yang, Jeffrey E. Pessin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

During phagocytosis, macrophages rapidly internalize a substantial fraction of plasma membrane without a net loss of surface area, suggesting that membranes are targeted to the cell surface from intracellular sites. Nevertheless, a requirement for mobilization of specific membrane compartments has not been demonstrated. We used bone marrow-derived macrophages (BMM) from wild type and vamp3 null mice to evaluate directly the requirement for this v-SNARE in phagocytosis of zymosan, IgG-beads, complement-opsonized particles, or latex microspheres. Regardless of the phagocytic receptor engaged or particle load, BMM lacking vamp3 exhibited no phagocytic defects when assayed after 1 h at 37°C, and phagosome maturation was unimpaired as judged by acquisition of lamp-1. In contrast, at early time points (5-15 min), internalization of zymosan (but not other particles tested) was significantly slower in vamp3 null BMM. These data indicate that vamp3 modulates efficient uptake of zymosan, but is not absolutely required for phagocytosis in primary macrophages.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalJournal of Leukocyte Biology
Volume72
Issue number1
StatePublished - Jul 1 2002
Externally publishedYes

Fingerprint

Phagocytosis
Macrophages
Zymosan
Microspheres
SNARE Proteins
Phagosomes
Membranes
Immunoglobulin G
Cell Membrane

Keywords

  • Cellubrevin
  • Exocytosis
  • IgG-beads
  • Phagosome
  • v-SNARE

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rate and extent of phagocytosis in macrophages lacking vamp3. / Allen, Lee Ann H; Yang, Chunmei; Pessin, Jeffrey E.

In: Journal of Leukocyte Biology, Vol. 72, No. 1, 01.07.2002, p. 217-221.

Research output: Contribution to journalArticle

Allen, Lee Ann H ; Yang, Chunmei ; Pessin, Jeffrey E. / Rate and extent of phagocytosis in macrophages lacking vamp3. In: Journal of Leukocyte Biology. 2002 ; Vol. 72, No. 1. pp. 217-221.
@article{0fedd575573f4c368fd7505a1eefa1b3,
title = "Rate and extent of phagocytosis in macrophages lacking vamp3",
abstract = "During phagocytosis, macrophages rapidly internalize a substantial fraction of plasma membrane without a net loss of surface area, suggesting that membranes are targeted to the cell surface from intracellular sites. Nevertheless, a requirement for mobilization of specific membrane compartments has not been demonstrated. We used bone marrow-derived macrophages (BMM) from wild type and vamp3 null mice to evaluate directly the requirement for this v-SNARE in phagocytosis of zymosan, IgG-beads, complement-opsonized particles, or latex microspheres. Regardless of the phagocytic receptor engaged or particle load, BMM lacking vamp3 exhibited no phagocytic defects when assayed after 1 h at 37°C, and phagosome maturation was unimpaired as judged by acquisition of lamp-1. In contrast, at early time points (5-15 min), internalization of zymosan (but not other particles tested) was significantly slower in vamp3 null BMM. These data indicate that vamp3 modulates efficient uptake of zymosan, but is not absolutely required for phagocytosis in primary macrophages.",
keywords = "Cellubrevin, Exocytosis, IgG-beads, Phagosome, v-SNARE",
author = "Allen, {Lee Ann H} and Chunmei Yang and Pessin, {Jeffrey E.}",
year = "2002",
month = "7",
day = "1",
language = "English (US)",
volume = "72",
pages = "217--221",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "1",

}

TY - JOUR

T1 - Rate and extent of phagocytosis in macrophages lacking vamp3

AU - Allen, Lee Ann H

AU - Yang, Chunmei

AU - Pessin, Jeffrey E.

PY - 2002/7/1

Y1 - 2002/7/1

N2 - During phagocytosis, macrophages rapidly internalize a substantial fraction of plasma membrane without a net loss of surface area, suggesting that membranes are targeted to the cell surface from intracellular sites. Nevertheless, a requirement for mobilization of specific membrane compartments has not been demonstrated. We used bone marrow-derived macrophages (BMM) from wild type and vamp3 null mice to evaluate directly the requirement for this v-SNARE in phagocytosis of zymosan, IgG-beads, complement-opsonized particles, or latex microspheres. Regardless of the phagocytic receptor engaged or particle load, BMM lacking vamp3 exhibited no phagocytic defects when assayed after 1 h at 37°C, and phagosome maturation was unimpaired as judged by acquisition of lamp-1. In contrast, at early time points (5-15 min), internalization of zymosan (but not other particles tested) was significantly slower in vamp3 null BMM. These data indicate that vamp3 modulates efficient uptake of zymosan, but is not absolutely required for phagocytosis in primary macrophages.

AB - During phagocytosis, macrophages rapidly internalize a substantial fraction of plasma membrane without a net loss of surface area, suggesting that membranes are targeted to the cell surface from intracellular sites. Nevertheless, a requirement for mobilization of specific membrane compartments has not been demonstrated. We used bone marrow-derived macrophages (BMM) from wild type and vamp3 null mice to evaluate directly the requirement for this v-SNARE in phagocytosis of zymosan, IgG-beads, complement-opsonized particles, or latex microspheres. Regardless of the phagocytic receptor engaged or particle load, BMM lacking vamp3 exhibited no phagocytic defects when assayed after 1 h at 37°C, and phagosome maturation was unimpaired as judged by acquisition of lamp-1. In contrast, at early time points (5-15 min), internalization of zymosan (but not other particles tested) was significantly slower in vamp3 null BMM. These data indicate that vamp3 modulates efficient uptake of zymosan, but is not absolutely required for phagocytosis in primary macrophages.

KW - Cellubrevin

KW - Exocytosis

KW - IgG-beads

KW - Phagosome

KW - v-SNARE

UR - http://www.scopus.com/inward/record.url?scp=0036658760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036658760&partnerID=8YFLogxK

M3 - Article

VL - 72

SP - 217

EP - 221

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 1

ER -