Rapid determination of membrane transport parameters in adherent cells

Karim A. Sharif, I. David Goldman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.

Original languageEnglish (US)
Pages (from-to)926-932
Number of pages7
JournalBioTechniques
Volume28
Issue number5
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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