Rapid determination of membrane transport parameters in adherent cells

Karim A. Sharif, I. David Goldman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.

Original languageEnglish (US)
Pages (from-to)926-932
Number of pages7
JournalBioTechniques
Volume28
Issue number5
StatePublished - 2000

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Monolayers
Reduced Folate Carrier Protein
Cells
Membranes
Scintillation
Substrates
Cricetulus
Methotrexate
Transport properties
Glass
Ovary
Breast Neoplasms
Costs and Cost Analysis
Cell Line
Costs

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rapid determination of membrane transport parameters in adherent cells. / Sharif, Karim A.; Goldman, I. David.

In: BioTechniques, Vol. 28, No. 5, 2000, p. 926-932.

Research output: Contribution to journalArticle

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