Radioimmunoassay of bovine heart protein kinase

N. Fleischer, O. M. Rosen, M. Reichlin

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Immunization of guinea pigs with bovine cardiac cAMP dependent protein kinase (ATP protein phosphotransferase, EC 2.7.1.37) resulted in the development of precipitating antibodies to the cAMP binding subunit of the enzyme. Both the phosphorylated and nonphosphorylated cAMP binding protein of the protein kinase reacted with the antiserum. A radioimmunoassay was developed that detects 10 ng of holoenzyme and permits measurement of enzyme concentrations in bovine cardiac muscle. Bovine liver, kidney, brain, and skeletal muscle contain protein kinases which are immunologically identical to those found in bovine cardiac muscle. However, the proportion of immunoreactive enzyme activity differed for each tissue. All of the immunologically nonreactive enzyme in skeletal muscle and heart was separable from immunoreactive enzyme by chromatography on DEAE cellulose. Rat tissues and pig heart contained protein kinase activity that crossreacted immunologically in a nonparallel fashion with bovine cardiac enzyme. These results indicate that cAMP dependent protein kinases within and between species are immunologically heterogeneous.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number1
StatePublished - 1976

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Protein Kinases
Radioimmunoassay
Enzymes
Cyclic AMP-Dependent Protein Kinases
Myocardium
Skeletal Muscle
DEAE-Cellulose Chromatography
Holoenzymes
Muscle Proteins
Immune Sera
Immunization
Carrier Proteins
Guinea Pigs
Swine
Kidney
Antibodies
Liver
Brain

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Radioimmunoassay of bovine heart protein kinase. / Fleischer, N.; Rosen, O. M.; Reichlin, M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 73, No. 1, 1976, p. 54-58.

Research output: Contribution to journalArticle

Fleischer, N. ; Rosen, O. M. ; Reichlin, M. / Radioimmunoassay of bovine heart protein kinase. In: Proceedings of the National Academy of Sciences of the United States of America. 1976 ; Vol. 73, No. 1. pp. 54-58.
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