Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice.

B. Paigen, N. J. Schork, K. L. Svenson, Y. C. Cheah, J. L. Mu, F. Lammert, David Q.H. Wang, G. Bouchard, M. C. Carey

Research output: Contribution to journalArticle

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Abstract

Quantitative trait locus (QTL) mapping was used to locate genes that determine the difference in cholesterol gallstone disease between the gallstone-susceptible strain C57L/J and the gallstone-resistant strain AKR/J. Gallstone weight was determined in 231 male (AKR x C57L) F(1) x AKR backcross mice fed a lithogenic diet containing 1% cholesterol, 0.5% cholic acid, and 15% butterfat for 8 wk. Mice having no stones and mice having the largest stones were genotyped at approximately 20-cM intervals to find the loci determining cholesterol gallstone formation. The major locus, Lith1, mapped near D2Mit56 and was confirmed by constructing a congenic strain, AK. L-Lith1(s). Another locus, Lith2, mapped near D19Mit58 and was also confirmed by constructing a congenic strain AK.L-Lith2(s). Other suggestive, but not statistically significant, loci mapped to chromosomes 6, 7, 8, 10, and X. The identification of these Lith genes will elucidate the pathophysiology of cholesterol gallstone formation.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalPhysiol Genomics
Volume4
Issue number1
StatePublished - Nov 9 2000
Externally publishedYes

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Quantitative Trait Loci
Gallstones
Cholesterol
Inbred AKR Mouse
Cholic Acid
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 7
Genes
Diet
Weights and Measures

ASJC Scopus subject areas

  • Genetics
  • Physiology

Cite this

Paigen, B., Schork, N. J., Svenson, K. L., Cheah, Y. C., Mu, J. L., Lammert, F., ... Carey, M. C. (2000). Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice. Physiol Genomics, 4(1), 59-65.

Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice. / Paigen, B.; Schork, N. J.; Svenson, K. L.; Cheah, Y. C.; Mu, J. L.; Lammert, F.; Wang, David Q.H.; Bouchard, G.; Carey, M. C.

In: Physiol Genomics, Vol. 4, No. 1, 09.11.2000, p. 59-65.

Research output: Contribution to journalArticle

Paigen, B, Schork, NJ, Svenson, KL, Cheah, YC, Mu, JL, Lammert, F, Wang, DQH, Bouchard, G & Carey, MC 2000, 'Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice.', Physiol Genomics, vol. 4, no. 1, pp. 59-65.
Paigen B, Schork NJ, Svenson KL, Cheah YC, Mu JL, Lammert F et al. Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice. Physiol Genomics. 2000 Nov 9;4(1):59-65.
Paigen, B. ; Schork, N. J. ; Svenson, K. L. ; Cheah, Y. C. ; Mu, J. L. ; Lammert, F. ; Wang, David Q.H. ; Bouchard, G. ; Carey, M. C. / Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice. In: Physiol Genomics. 2000 ; Vol. 4, No. 1. pp. 59-65.
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