Quantitative Peptidomics of Purkinje Cell Degeneration Mice

Iryna Berezniuk, Juan J. Sironi, Jonathan Wardman, Raymond C. Pasek, Nicolas F. Berbari, Bradley K. Yoder, Lloyd D. Fricker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cytosolic carboxypeptidase 1 (CCP1) is a metallopeptidase that removes C-terminal and side-chain glutamates from tubulin. The Purkinje cell degeneration (pcd) mouse lacks CCP1 due to a mutation. Previously, elevated levels of peptides derived from cytosolic and mitochondrial proteins were found in adult pcd mouse brain, raising the possibility that CCP1 functions in the degradation of intracellular peptides. To test this hypothesis, we used a quantitative peptidomics technique to compare peptide levels in wild-type and pcd mice, examining adult heart, spleen, and brain, and presymptomatic 3 week-old amygdala and cerebellum. Contrary to adult mouse brain, young pcd brain and adult heart and spleen did not show a large increase in levels of intracellular peptides. Unexpectedly, levels of peptides derived from secretory pathway proteins were altered in adult pcd mouse brain. The pattern of changes for the intracellular and secretory pathway peptides in pcd mice was generally similar to the pattern observed in mice lacking primary cilia. Collectively, these results suggest that intracellular peptide accumulation in adult pcd mouse brain is a secondary effect and is not due to a role of CCP1 in peptide turnover.

Original languageEnglish (US)
Article numbere60981
JournalPLoS One
Volume8
Issue number4
DOIs
StatePublished - Apr 8 2013

Fingerprint

Purkinje Cells
peptides
Carboxypeptidases
carboxypeptidases
Brain
Peptides
mice
brain
cells
Secretory Pathway
spleen
Spleen
heart
Glutamates
amygdala
Cilia
Mitochondrial Proteins
Metalloproteases
metalloproteinases
cilia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Berezniuk, I., Sironi, J. J., Wardman, J., Pasek, R. C., Berbari, N. F., Yoder, B. K., & Fricker, L. D. (2013). Quantitative Peptidomics of Purkinje Cell Degeneration Mice. PLoS One, 8(4), [e60981]. https://doi.org/10.1371/journal.pone.0060981

Quantitative Peptidomics of Purkinje Cell Degeneration Mice. / Berezniuk, Iryna; Sironi, Juan J.; Wardman, Jonathan; Pasek, Raymond C.; Berbari, Nicolas F.; Yoder, Bradley K.; Fricker, Lloyd D.

In: PLoS One, Vol. 8, No. 4, e60981, 08.04.2013.

Research output: Contribution to journalArticle

Berezniuk, I, Sironi, JJ, Wardman, J, Pasek, RC, Berbari, NF, Yoder, BK & Fricker, LD 2013, 'Quantitative Peptidomics of Purkinje Cell Degeneration Mice', PLoS One, vol. 8, no. 4, e60981. https://doi.org/10.1371/journal.pone.0060981
Berezniuk I, Sironi JJ, Wardman J, Pasek RC, Berbari NF, Yoder BK et al. Quantitative Peptidomics of Purkinje Cell Degeneration Mice. PLoS One. 2013 Apr 8;8(4). e60981. https://doi.org/10.1371/journal.pone.0060981
Berezniuk, Iryna ; Sironi, Juan J. ; Wardman, Jonathan ; Pasek, Raymond C. ; Berbari, Nicolas F. ; Yoder, Bradley K. ; Fricker, Lloyd D. / Quantitative Peptidomics of Purkinje Cell Degeneration Mice. In: PLoS One. 2013 ; Vol. 8, No. 4.
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