Quantitative peptidomics of mice lacking peptide-processing enzymes

Jonathan Wardman, Lloyd D. Fricker

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Citations (Scopus)

Abstract

Peptidomics is defined as the analysis of peptides present in a tissue extract, usually using mass spectrometry-based approaches. Unlike radioimmunoassay-based detection techniques, peptidomics measures the precise form of each peptide, including post-translational modifications, and can readily distinguish between longer and shorter forms of the same peptide. Also, peptidomics is not limited to known peptides and can detect hundreds of peptides in a single experiment. Quantitative peptidomics enables comparisons between two or more groups of samples and is perfect for studies examining the effect of gene knockouts on tissue levels of peptides. We describe the method for quantitative peptidomics using isotopic labels based on trimethylammonium butyrate, which can be synthesized in five different isotopic forms; this permits multivariate analysis of five different groups of tissue extracts in a single liquid chromatography/mass spectrometry run.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages307-323
Number of pages17
Volume768
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume768
ISSN (Print)10643745

Fingerprint

Peptides
Enzymes
Tissue Extracts
Mass Spectrometry
Gene Knockout Techniques
Butyrates
Post Translational Protein Processing
Liquid Chromatography
Radioimmunoassay
Multivariate Analysis

Keywords

  • carboxypeptidase
  • peptidase
  • peptidomics
  • Prohormone convertase
  • proprotein convertase
  • protease
  • proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wardman, J., & Fricker, L. D. (2011). Quantitative peptidomics of mice lacking peptide-processing enzymes. In Methods in Molecular Biology (Vol. 768, pp. 307-323). (Methods in Molecular Biology; Vol. 768). https://doi.org/10.1007/978-1-61779-204-5_17

Quantitative peptidomics of mice lacking peptide-processing enzymes. / Wardman, Jonathan; Fricker, Lloyd D.

Methods in Molecular Biology. Vol. 768 2011. p. 307-323 (Methods in Molecular Biology; Vol. 768).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wardman, J & Fricker, LD 2011, Quantitative peptidomics of mice lacking peptide-processing enzymes. in Methods in Molecular Biology. vol. 768, Methods in Molecular Biology, vol. 768, pp. 307-323. https://doi.org/10.1007/978-1-61779-204-5_17
Wardman J, Fricker LD. Quantitative peptidomics of mice lacking peptide-processing enzymes. In Methods in Molecular Biology. Vol. 768. 2011. p. 307-323. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-204-5_17
Wardman, Jonathan ; Fricker, Lloyd D. / Quantitative peptidomics of mice lacking peptide-processing enzymes. Methods in Molecular Biology. Vol. 768 2011. pp. 307-323 (Methods in Molecular Biology).
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