Quantitative peptidomics of mice lacking peptide-processing enzymes

Jonathan Wardman, Lloyd D. Fricker

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Scopus citations

Abstract

Peptidomics is defined as the analysis of peptides present in a tissue extract, usually using mass spectrometry-based approaches. Unlike radioimmunoassay-based detection techniques, peptidomics measures the precise form of each peptide, including post-translational modifications, and can readily distinguish between longer and shorter forms of the same peptide. Also, peptidomics is not limited to known peptides and can detect hundreds of peptides in a single experiment. Quantitative peptidomics enables comparisons between two or more groups of samples and is perfect for studies examining the effect of gene knockouts on tissue levels of peptides. We describe the method for quantitative peptidomics using isotopic labels based on trimethylammonium butyrate, which can be synthesized in five different isotopic forms; this permits multivariate analysis of five different groups of tissue extracts in a single liquid chromatography/mass spectrometry run.

Original languageEnglish (US)
Title of host publicationProprotein Convertases
EditorsMajambu Mbikay, Nabil G. Seidah
Pages307-323
Number of pages17
DOIs
StatePublished - Oct 24 2011

Publication series

NameMethods in Molecular Biology
Volume768
ISSN (Print)1064-3745

Keywords

  • Prohormone convertase
  • carboxypeptidase
  • peptidase
  • peptidomics
  • proprotein convertase
  • protease
  • proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wardman, J., & Fricker, L. D. (2011). Quantitative peptidomics of mice lacking peptide-processing enzymes. In M. Mbikay, & N. G. Seidah (Eds.), Proprotein Convertases (pp. 307-323). (Methods in Molecular Biology; Vol. 768). https://doi.org/10.1007/978-1-61779-204-5_17