Quantitative mRNA imaging throughout the entire Drosophila brain

Xi Long, Jennifer Colonell, Allan M. Wong, Robert H. Singer, Timothée Lionnet

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).

Original languageEnglish (US)
Pages (from-to)703-706
Number of pages4
JournalNature Methods
Volume14
Issue number7
DOIs
StatePublished - Jun 29 2017

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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