Quantitative mRNA imaging throughout the entire Drosophila brain

Xi Long, Jennifer Colonell, Allan M. Wong, Robert H. Singer, Timothée Lionnet

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).

Original languageEnglish (US)
Pages (from-to)703-706
Number of pages4
JournalNature Methods
Volume14
Issue number7
DOIs
StatePublished - Jun 29 2017

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Drosophila
Brain
Imaging techniques
Messenger RNA
Microscopes
Lighting
Fluorescence In Situ Hybridization
Amplification
Fluorescence

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

Quantitative mRNA imaging throughout the entire Drosophila brain. / Long, Xi; Colonell, Jennifer; Wong, Allan M.; Singer, Robert H.; Lionnet, Timothée.

In: Nature Methods, Vol. 14, No. 7, 29.06.2017, p. 703-706.

Research output: Contribution to journalArticle

Long, X, Colonell, J, Wong, AM, Singer, RH & Lionnet, T 2017, 'Quantitative mRNA imaging throughout the entire Drosophila brain', Nature Methods, vol. 14, no. 7, pp. 703-706. https://doi.org/10.1038/nmeth.4309
Long, Xi ; Colonell, Jennifer ; Wong, Allan M. ; Singer, Robert H. ; Lionnet, Timothée. / Quantitative mRNA imaging throughout the entire Drosophila brain. In: Nature Methods. 2017 ; Vol. 14, No. 7. pp. 703-706.
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