Putative proteins involved in manganese transport across the blood-brain barrier

Vanessa A. Fitsanakis, Greg Piccola, Ana Paula Marreilha Dos Santos, Judy L. Aschner, Michael Aschner

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Manganese (Mn) is an essential nutrient required for proper growth and maintenance of numerous biological systems. At high levels it is known to be neurotoxic. While focused research concerning the transport of Mn across the blood-brain barrier (BBB) is on-going, the exact identity of the transporter(s) responsible is still debated. The transferrin receptor (TfR) and the divalent metal transporter-1 (DMT-1) have long been thought to play a role in brain Mn deposition. However, evidence suggests that Mn may also be transported by other proteins. One model system of the BBB, rat brain endothelial (RBE4) cells, are known to express many proteins suspected to be involved in metal transport. This review will discuss the biological importance of Mn, and then briefly describe several proteins that may be involved in transport of this metal across the BBB. The latter section will examine the potential usefulness of RBE4 cells in characterizing various aspects of Mn transport, and basic culture techniques involved in working with these cells. It is hoped that ideas put forth in this article will stimulate further investigations into the complex nature of Mn transport, and address the importance as well as the limitation of in vitro models in answering these questions.

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalHuman and Experimental Toxicology
Volume26
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

Manganese
Blood-Brain Barrier
Proteins
Brain
Metals
Culture Techniques
Transferrin Receptors
Endothelial cells
Biological systems
Nutrients
Rats
Endothelial Cells
Food
Growth
Research

Keywords

  • In vitro cell culture
  • Manganese
  • Metal transporters
  • Rat brain endothelial cells (RBE4)

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Putative proteins involved in manganese transport across the blood-brain barrier. / Fitsanakis, Vanessa A.; Piccola, Greg; Dos Santos, Ana Paula Marreilha; Aschner, Judy L.; Aschner, Michael.

In: Human and Experimental Toxicology, Vol. 26, No. 4, 04.2007, p. 295-302.

Research output: Contribution to journalArticle

Fitsanakis, Vanessa A. ; Piccola, Greg ; Dos Santos, Ana Paula Marreilha ; Aschner, Judy L. ; Aschner, Michael. / Putative proteins involved in manganese transport across the blood-brain barrier. In: Human and Experimental Toxicology. 2007 ; Vol. 26, No. 4. pp. 295-302.
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