Purinergic glio-endothelial coupling during neuronal activity

Role of P2Y1 receptors and eNOS in functional hyperemia in the mouse somatosensory cortex

Peter Toth, Stefano Tarantini, Antonio Davila, M. Noa Valcarcel-Ares, Zsuzsanna Tucsek, Behzad Varamini, Praveen Ballabh, William E. Sonntag, Joseph A. Baur, Anna Csiszar, Zoltan Ungvari

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Impairment of moment-tomoment adjustment of cerebral blood flow (CBF) via neurovascular coupling is thought to play a critical role in the genesis of cognitive impairment associated with aging and pathological conditions associated with accelerated cerebromicrovascular aging (e.g., hypertension, obesity). Although previous studies demonstrate that endothelial dysfunction plays a critical role in neurovascular uncoupling in these conditions, the role of endothelial NO mediation in neurovascular coupling responses is not well understood. To establish the link between endothelial function and functional hyperemia, neurovascular coupling responses were studied in mutant mice overexpressing or deficient in endothelial NO synthase (eNOS), and the role of P2Y1 receptors in purinergic glioendothelial coupling was assessed. We found that genetic depletion of eNOS (eNOS-/-) and pharmacological inhibition of NO synthesis significantly decreased the CBF responses in the somatosensory cortex evoked by whisker stimulation and by administration of ATP. Overexpression of eNOS enhanced NO mediation of functional hyperemia. In control mice, the selective and potent P2Y1 receptor antagonist MRS2179 attenuated both whisker stimulation-induced and ATP-mediated CBF responses, whereas, in eNOS-/- mice, the inhibitory effects of MRS2179 were blunted. Collectively, our findings provide additional evidence for purinergic glio-endothelial coupling during neuronal activity, highlighting the role of ATP-mediated activation of eNOS via P2Y1 receptors in functional hyperemia.

Original languageEnglish (US)
Pages (from-to)H1837-H1845
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume309
Issue number11
DOIs
StatePublished - 2015
Externally publishedYes

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Purinergic P2Y1 Receptors
Somatosensory Cortex
Hyperemia
Cerebrovascular Circulation
Nitric Oxide Synthase
Vibrissae
Adenosine Triphosphate
Social Adjustment
Obesity
Pharmacology
Hypertension
Neurovascular Coupling

Keywords

  • Astrocyte
  • Dementia
  • Endothelial dysfunction
  • Endothelial nitric oxide synthase
  • Endothelium
  • Vascular cognitive impairment

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Purinergic glio-endothelial coupling during neuronal activity : Role of P2Y1 receptors and eNOS in functional hyperemia in the mouse somatosensory cortex. / Toth, Peter; Tarantini, Stefano; Davila, Antonio; Noa Valcarcel-Ares, M.; Tucsek, Zsuzsanna; Varamini, Behzad; Ballabh, Praveen; Sonntag, William E.; Baur, Joseph A.; Csiszar, Anna; Ungvari, Zoltan.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 309, No. 11, 2015, p. H1837-H1845.

Research output: Contribution to journalArticle

Toth, Peter ; Tarantini, Stefano ; Davila, Antonio ; Noa Valcarcel-Ares, M. ; Tucsek, Zsuzsanna ; Varamini, Behzad ; Ballabh, Praveen ; Sonntag, William E. ; Baur, Joseph A. ; Csiszar, Anna ; Ungvari, Zoltan. / Purinergic glio-endothelial coupling during neuronal activity : Role of P2Y1 receptors and eNOS in functional hyperemia in the mouse somatosensory cortex. In: American Journal of Physiology - Heart and Circulatory Physiology. 2015 ; Vol. 309, No. 11. pp. H1837-H1845.
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