Pseudorabies virus and herpes simplex virus type 1 utilize different tegument-glycoprotein interactions to mediate the process of envelopment

Omar S. Omar, Alicia J. Simmons, Nicole M. Andre, Duncan W. Wilson, Sarah T. Gross

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background and Objective: During herpesvirus envelopment capsids, tegument polypeptides and membrane proteins assemble at the site of budding, and a cellular lipid bilayer becomes refashioned into a spherical envelope. A web of interactions between tegument proteins and the cytoplasmic tails of viral glycoproteins play a critical role in this process. We have previously demonstrated that for herpes simplex virus (HSV)-1 the cytoplasmic tail of glycoprotein H (gH) binds the tegument protein VP16. The HSV and pseudorabies virus (PRV) genomes are essentially collinear, and individual gene products show significant sequence homology. However, the demarcation of function often differs between PRV and HSV proteins. The goal of this study was to determine whether PRV gH and VP16 interact in a manner similar to their homologs in HSV. Methods: A fusion protein pull-down assay was performed in which a PRV gH cytoplasmic tail-glutathione S-transferase fusion protein, bound to glutathione-Sepharose beads, was incubated with PRV-infected cell cytosol, washed and subjected to Western blot analysis using anti-PRV VP16 antisera. Results: Western blots indicate that PRV VP16 does not specifically bind to the PRV gH tail. Conclusion: Our results highlight that, despite the relatively close evolutionary relationship between HSV and PRV, there are significant differences in their protein interactions that drive envelopment.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalIntervirology
Volume56
Issue number1
DOIs
StatePublished - Nov 1 2012

Keywords

  • Envelopment
  • Glycoprotein
  • HSV-1
  • PRV
  • Tegument
  • VP16
  • gH

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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