Proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores

Supriya Pai, Andrew D. Ellington, Matthew Levy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The proximity ligation assay (PLA) has previously been used for the sensitive and specific detection of single proteins. In order to adapt PLA methods for the detection of cell surfaces, we have generated multivalent peptide-oligonucleotide-phycoerythrin conjugates ('burrs') that can bind adjacent to one another on a cell surface and be ligated together to form unique amplicons. Real-time PCR detection of burr ligation events specifically identified as few as 100 Bacillus anthracis, 10 Bacillus subtilis and 1 Bacillus cereus spore. Burrs should prove to be generally useful for detecting and mapping interactions and distances between cell surface proteins.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalNucleic Acids Research
Volume33
Issue number18
DOIs
StatePublished - 2005
Externally publishedYes

Fingerprint

Spores
Ligation
Peptides
Phycoerythrin
Bacillus anthracis
Bacillus cereus
Bacillus subtilis
Oligonucleotides
Real-Time Polymerase Chain Reaction
Membrane Proteins
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores. / Pai, Supriya; Ellington, Andrew D.; Levy, Matthew.

In: Nucleic Acids Research, Vol. 33, No. 18, 2005, p. 1-7.

Research output: Contribution to journalArticle

Pai, Supriya ; Ellington, Andrew D. ; Levy, Matthew. / Proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores. In: Nucleic Acids Research. 2005 ; Vol. 33, No. 18. pp. 1-7.
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