@article{61e7b94e931243579f66c2f44190723a,
title = "Proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores",
abstract = "The proximity ligation assay (PLA) has previously been used for the sensitive and specific detection of single proteins. In order to adapt PLA methods for the detection of cell surfaces, we have generated multivalent peptide-oligonucleotide-phycoerythrin conjugates ('burrs') that can bind adjacent to one another on a cell surface and be ligated together to form unique amplicons. Real-time PCR detection of burr ligation events specifically identified as few as 100 Bacillus anthracis, 10 Bacillus subtilis and 1 Bacillus cereus spore. Burrs should prove to be generally useful for detecting and mapping interactions and distances between cell surface proteins.",
author = "Supriya Pai and Ellington, {Andrew D.} and Matthew Levy",
note = "Funding Information: We thank Dr Charles L. Turnbough Jr for providing us with the sequences of the spore binding peptides as well as additional assistance on this project. We also thank Megan Breeden, for her assistance in BA spore preparation and microscopy, Seth Cockrum for his assistance with BS and BC spore preparation and Brad Hall for his assistance with real-time PCR. This work was supported by a grant from HSARP and Accacia International (sub award UTA04-419) and a grant from the Army Research Office and Echo Technical (sub award UTA05-006). Funding to pay the Open Access publication charges for this article was provided by Department Support Funds.",
year = "2005",
doi = "10.1093/nar/gni150",
language = "English (US)",
volume = "33",
pages = "1--7",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "18",
}