Proteomic characterization of a mouse model of familial danish dementia

Monica Vitale, Giovanni Renzone, Shuji Matsuda, Andrea Scaloni, Luciano D'Adamio, Nicola Zambrano

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A dominant mutation in the ITM2B/BRI2 gene causes familial Danish dementia (FDD) in humans. To model FDD in animal systems, a knock-in approach was recently implemented in mice expressing a wild-type and mutant allele, which bears the FDD-associated mutation. Since these FDD KI mice show behavioural alterations and impaired synaptic function, we characterized their synaptosomal proteome via two-dimensional differential in-gel electrophoresis. After identification by nanoliquid chromatography coupled to electrospray-linear ion trap tandem mass spectrometry, the differentially expressed proteins were classified according to their gene ontology descriptions and their predicted functional interactions. The Dlg4/Psd95 scaffold protein and additional signalling proteins, including protein phosphatases, were revealed by STRING analysis as potential players in the altered synaptic function of FDD KI mice. Immunoblotting analysis finally demonstrated the actual downregulation of the synaptosomal scaffold protein Dlg4/Psd95 and of the dual-specificity phosphatase Dusp3 in the synaptosomes of FDD KI mice.

Original languageEnglish (US)
Article number728178
JournalJournal of Biomedicine and Biotechnology
Volume2012
DOIs
StatePublished - 2012

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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    Vitale, M., Renzone, G., Matsuda, S., Scaloni, A., D'Adamio, L., & Zambrano, N. (2012). Proteomic characterization of a mouse model of familial danish dementia. Journal of Biomedicine and Biotechnology, 2012, [728178]. https://doi.org/10.1155/2012/728178