Protein production and purification

Susanne Gräslund, Pär Nordlund, Johan Weigelt, B. Martin Hallberg, James Bray, Opher Gileadi, Stefan Knapp, Udo Oppermann, Cheryl Arrowsmith, Raymond Hui, Jinrong Ming, Sirano dhe-Paganon, Hee Won Park, Alexei Savchenko, Adelinda Yee, Aled Edwards, Renaud Vincentelli, Christian Cambillau, Rosalind Kim, Sung Hou KimZihe Rao, Yunyu Shi, Thomas C. Terwilliger, Chang Yub Kim, Li Wei Hung, Geoffrey S. Waldo, Yoav Peleg, Shira Albeck, Tamar Unger, Orly Dym, Jaime Prilusky, Joel L. Sussman, Ray C. Stevens, Scott A. Lesley, Ian A. Wilson, Andrzej Joachimiak, Frank Collart, Irina Dementieva, Mark I. Donnelly, William H. Eschenfeldt, Youngchang Kim, Lucy Stols, Ruying Wu, Min Zhou, Stephen K. Burley, J. Spencer Emtage, J. Michael Sauder, Devon Thompson, Kevin Bain, John Luz, Tarun Gheyi, Fred Zhang, Shane Atwell, Steven C. Almo, Jeffrey B. Bonanno, Andras Fiser, Sivasubramanian Swaminathan, F. William Studier, Mark R. Chance, Andrej Sali, Thomas B. Acton, Rong Xiao, Li Zhao, Li Chung Ma, John F. Hunt, Liang Tong, Kellie Cunningham, Masayori Inouye, Stephen Anderson, Heleema Janjua, Ritu Shastry, Chi Kent Ho, Dongyan Wang, Huang Wang, Mei Jiang, Gaetano T. Montelione, David I. Stuart, Raymond J. Owens, Susan Daenke, Anja Schütz, Udo Heinemann, Shigeyuki Yokoyama, Konrad Büssow, Kristin C. Gunsalus

Research output: Contribution to journalReview article

539 Scopus citations

Abstract

In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

Original languageEnglish (US)
Pages (from-to)135-146
Number of pages12
JournalNature Methods
Volume5
Issue number2
DOIs
StatePublished - Feb 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M., Bray, J., Gileadi, O., Knapp, S., Oppermann, U., Arrowsmith, C., Hui, R., Ming, J., dhe-Paganon, S., Park, H. W., Savchenko, A., Yee, A., Edwards, A., Vincentelli, R., Cambillau, C., Kim, R., ... Gunsalus, K. C. (2008). Protein production and purification. Nature Methods, 5(2), 135-146. https://doi.org/10.1038/nmeth.f.202