Protein-induced bending or flexing at the 5′-end of the duck βA-globin promoter

Romana Mátlová, Květa Horská, Vladimir Benes, Aleš Cvekl, Jaroslav Sponar

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The 5′-end of the duck βA-globin promoter contains a protein binding site BS-3/Spl, the A + T-rich part of which could be involved in DNA bending. Plasmids were constructed using the pBend2 plasmid containing BS-3/Spl. Circular permutation analysis of the fragments cut out from the plasmids using various restriction endonucleases, in the presence of a partially purified protein extract from embryonic duck erythrocytes, was performed. The results indicate that a rather complicated change in the fragment shape takes place upon protein binding, which is best explained as an induction of two points of bending or flexure within the fragment. Analogical points of flexure may exist at the protein-binding sites of the duck and chicken βA-globin promoters in spite of differing DNA sequences.

Original languageEnglish (US)
Pages (from-to)277-281
Number of pages5
JournalGene
Volume156
Issue number2
DOIs
StatePublished - Apr 24 1995
Externally publishedYes

Keywords

  • DNA bending
  • Spl-binding site
  • circular permutation analysis
  • embryonic duck erythrocytes

ASJC Scopus subject areas

  • Genetics

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