Prostaglandin synthesis in isolated glomeruli

Vaughn W. Folkert, Detlef Schlondorff

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Prostaglandins are thought to play an important role in the local regulation of glomerular blood flow and in the release of renin from the juxtaglomerular apparatus. We therefore examined prostaglandin synthesis by isolated rat glomeruli. Isolated glomeruli were either prelabeled with [14C] arachidonic acid or were incubated with [14C] arachidonic acid for the entire experimental incubation in Krebs buffer. Prostaglandin synthesis was determined by thin layer radiochromatography of acid extracts of the supernatant solutions. Indomethacin inhibitable synthesis of small amounts of 6-keto-PGF, the metabolite of prostacyclin(PGI2,) and larger amounts of PGF, and PGE2, and possibly thromboxane B2 (TXB2) by isolated glomeruli could be demonstrated with either prelabeling or direct incubation. These findings support the hypothesis that prostaglandins are produced within the glomerulus where they may affect local glomerular blood flow and function.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalProstaglandins
Volume17
Issue number1
DOIs
StatePublished - Jan 1979

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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