Prostaglandin synthesis in isolated glomeruli

Vaughn Wesley Folkert, Detlef Schlondorff

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Prostaglandins are thought to play an important role in the local regulation of glomerular blood flow and in the release of renin from the juxtaglomerular apparatus. We therefore examined prostaglandin synthesis by isolated rat glomeruli. Isolated glomeruli were either prelabeled with [14C] arachidonic acid or were incubated with [14C] arachidonic acid for the entire experimental incubation in Krebs buffer. Prostaglandin synthesis was determined by thin layer radiochromatography of acid extracts of the supernatant solutions. Indomethacin inhibitable synthesis of small amounts of 6-keto-PGF, the metabolite of prostacyclin(PGI2,) and larger amounts of PGF, and PGE2, and possibly thromboxane B2 (TXB2) by isolated glomeruli could be demonstrated with either prelabeling or direct incubation. These findings support the hypothesis that prostaglandins are produced within the glomerulus where they may affect local glomerular blood flow and function.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalProstaglandins
Volume17
Issue number1
DOIs
StatePublished - 1979

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Prostaglandins
Epoprostenol
Arachidonic Acid
Blood
Juxtaglomerular Apparatus
Thromboxane B2
Dinoprost
Metabolites
Dinoprostone
Renin
Indomethacin
Rats
Buffers
Acids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Prostaglandin synthesis in isolated glomeruli. / Folkert, Vaughn Wesley; Schlondorff, Detlef.

In: Prostaglandins, Vol. 17, No. 1, 1979, p. 79-86.

Research output: Contribution to journalArticle

Folkert, Vaughn Wesley ; Schlondorff, Detlef. / Prostaglandin synthesis in isolated glomeruli. In: Prostaglandins. 1979 ; Vol. 17, No. 1. pp. 79-86.
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