Prospective cohort study of the circadian rhythm pattern in allogeneic sibling donors undergoing standard granulocyte colony-stimulating factor mobilization

Patricia A. Shi, Luis M. Isola, Janice L. Gabrilove, Erin L. Moshier, James H. Godbold, Lorraine K. Miller, Paul S. Frenette

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction. Prior in vivo murine studies suggest circadian oscillations for hematopoietic stem cell release, which are maintained following administration of granulocyte colony-stimulating factor (G-CSF) or plerixafor. Furthermore, retrospective data analysis of healthy donors who underwent G-CSF-induced mobilization demonstrated significantly increased CD34+ cell yields when collected in the afternoon compared with the morning. Methods. A prospective study was conducted to directly examine the number of peripheral blood CD34+ and CD34+CD38- progenitor/stem cells at baseline and then every 6 hours for 24 hours on days 4 to 5 of G-CSF (10 μg/kg/day in the morning) mobilization in 11 allogeneic donors. Data were analyzed using mixed-model analysis of repeated measures. Results: Whereas we observed a significant increase in CD34+ cell counts toward the evening, counts were then sustained on the morning of day 5. The correlation between CD34+CD38- cell counts and the less defined CD34+ populations was weak. Conclusions: Our results suggest that the pharmacodynamic activity and timing of G-CSF may alter endogenous progenitor rhythms. Donor age, medical history, and medications may also impact circadian rhythm. Further studies should examine the circadian rhythm at the peak of G-CSF mobilization and should consider potential confounders such as the time of G-CSF administration and the age of the subjects.

Original languageEnglish (US)
Article number30
JournalStem Cell Research and Therapy
Volume4
Issue number2
DOIs
StatePublished - 2013

Fingerprint

Granulocyte Colony-Stimulating Factor
Circadian Rhythm
Cohort Studies
Prospective Studies
Stem cells
Stem Cells
Cell Count
Pharmacodynamics
Hematopoietic Stem Cells
Blood
Population

Keywords

  • Antigens
  • CD34
  • CD38
  • Circadian rhythm
  • Granulocyte-colony stimulating factor
  • Hematopoietic progenitor cells
  • Hematopoietic stem cell mobilization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)
  • Medicine(all)

Cite this

Prospective cohort study of the circadian rhythm pattern in allogeneic sibling donors undergoing standard granulocyte colony-stimulating factor mobilization. / Shi, Patricia A.; Isola, Luis M.; Gabrilove, Janice L.; Moshier, Erin L.; Godbold, James H.; Miller, Lorraine K.; Frenette, Paul S.

In: Stem Cell Research and Therapy, Vol. 4, No. 2, 30, 2013.

Research output: Contribution to journalArticle

Shi, Patricia A. ; Isola, Luis M. ; Gabrilove, Janice L. ; Moshier, Erin L. ; Godbold, James H. ; Miller, Lorraine K. ; Frenette, Paul S. / Prospective cohort study of the circadian rhythm pattern in allogeneic sibling donors undergoing standard granulocyte colony-stimulating factor mobilization. In: Stem Cell Research and Therapy. 2013 ; Vol. 4, No. 2.
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