Promiscuity of MHC Class Ib-Restricted T Cell Responses

Alexander Ploss, Gregoire Lauvau, Brian Contos, Kristen M. Kerksiek, Patrick D. Guirnalda, Ingrid Leiner, Laurel L. Lenz, Michael J. Bevan, Eric G. Pamer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Murine infection with the Gram-positive intracellular bacterium Listeria monocytogenes activates CD8+ T cells that recognize bacterially derived N-formyl methionine peptides in the context of H2-M3 MHC class Ib molecules. Three peptides, fMIGWII, fMIVIL, and fMIVTLF, are targets of L. monocytogenes-specific CD8+ T cells. To investigate epitope cross-recognition by H2-M3-restricted CD8+ T cells, we deleted the sequence encoding fMIGWII from a virulent strain of L. monocytogenes. Infection with fMIGWII-deficient L. monocytogenes unexpectedly primed CD8+ T cells that stain with fMIGWII/H2-M3 tetramers and lyse fMIGWII-coated target cells in vivo. Because the fMIGWII sequence is nonredundant, we speculated that other bacterially derived Ags are priming these responses. HPLC peptide fractionation of bacterial culture supernatants revealed several distinct L. monocytogenes-derived peptides that are recognized by fMIGWII-specific T cells. Our results demonstrate that the dominant H2-M3-restricted CD8+ T cell population, although reactive with fMIGWII, is primed by other, non-fMIGWII peptides derived from L. monocytogenes. Although this degree of Ag receptor promiscuity is unusual for the adaptive immune system, it may be a more common feature of T cell responses restricted by nonpolymorphic MHC class Ib molecules.

Original languageEnglish (US)
Pages (from-to)5948-5955
Number of pages8
JournalJournal of Immunology
Volume171
Issue number11
StatePublished - Dec 1 2003
Externally publishedYes

Fingerprint

Listeria monocytogenes
T-Lymphocytes
Peptides
Gram-Positive Bacteria
Infection
Methionine
Epitopes
Immune System
Coloring Agents
High Pressure Liquid Chromatography
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Ploss, A., Lauvau, G., Contos, B., Kerksiek, K. M., Guirnalda, P. D., Leiner, I., ... Pamer, E. G. (2003). Promiscuity of MHC Class Ib-Restricted T Cell Responses. Journal of Immunology, 171(11), 5948-5955.

Promiscuity of MHC Class Ib-Restricted T Cell Responses. / Ploss, Alexander; Lauvau, Gregoire; Contos, Brian; Kerksiek, Kristen M.; Guirnalda, Patrick D.; Leiner, Ingrid; Lenz, Laurel L.; Bevan, Michael J.; Pamer, Eric G.

In: Journal of Immunology, Vol. 171, No. 11, 01.12.2003, p. 5948-5955.

Research output: Contribution to journalArticle

Ploss, A, Lauvau, G, Contos, B, Kerksiek, KM, Guirnalda, PD, Leiner, I, Lenz, LL, Bevan, MJ & Pamer, EG 2003, 'Promiscuity of MHC Class Ib-Restricted T Cell Responses', Journal of Immunology, vol. 171, no. 11, pp. 5948-5955.
Ploss A, Lauvau G, Contos B, Kerksiek KM, Guirnalda PD, Leiner I et al. Promiscuity of MHC Class Ib-Restricted T Cell Responses. Journal of Immunology. 2003 Dec 1;171(11):5948-5955.
Ploss, Alexander ; Lauvau, Gregoire ; Contos, Brian ; Kerksiek, Kristen M. ; Guirnalda, Patrick D. ; Leiner, Ingrid ; Lenz, Laurel L. ; Bevan, Michael J. ; Pamer, Eric G. / Promiscuity of MHC Class Ib-Restricted T Cell Responses. In: Journal of Immunology. 2003 ; Vol. 171, No. 11. pp. 5948-5955.
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