Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis

Xiao Xia Wang, Robert J. Allen, John Paul Tutela, Alexander Sailon, Alexander C. Allori, Edward H. Davidson, Gina K. Paek, Pierre B. Saadeh, Joseph G. McCarthy, Stephen M. Warren

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Although bone repair is a relatively efficient process, a significant portion of patients fail to heal their fractures. Because adequate blood supply is essential to osteogenesis, the authors hypothesize that augmenting neovascularization by increasing the number of circulating progenitor cells will improve bony healing. Methods: Bilateral full-thickness defects were created in the parietal bones of C57 wild-type mice. Intraperitoneal AMD3100 (n ≤ 33) or sterile saline (n ≤ 33) was administered daily beginning on postoperative day 3 and continuing through day 18. Circulating progenitor cell number was quantified by fluorescence-activated cell sorting. Bone regeneration was assessed with micro-computed tomography. Immunofluorescent CD31 and osteocalcin staining was performed to assess for vascularity and osteoblast density. Results: AMD3100 treatment increased circulating progenitor cell levels and significantly improved bone regeneration. Calvarial defects of AMD3100-treated mice demonstrated increased vascularity and osteoblast density. Conclusions: Improved bone regeneration in this model was associated with elevated circulating progenitor cell number and subsequently improved neovascularization and osteogenesis. These findings highlight the importance of circulating progenitor cells in bone healing and may provide a novel therapy for bone regeneration.

Original languageEnglish (US)
Pages (from-to)395-405
Number of pages11
JournalPlastic and Reconstructive Surgery
Volume128
Issue number2
DOIs
StatePublished - Aug 1 2011
Externally publishedYes

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Osteogenesis
Bone Regeneration
Stem Cells
Bone and Bones
Osteoblasts
Cell Count
Parietal Bone
Osteocalcin
Flow Cytometry
Tomography
Staining and Labeling
Therapeutics
JM 3100

ASJC Scopus subject areas

  • Surgery

Cite this

Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis. / Wang, Xiao Xia; Allen, Robert J.; Tutela, John Paul; Sailon, Alexander; Allori, Alexander C.; Davidson, Edward H.; Paek, Gina K.; Saadeh, Pierre B.; McCarthy, Joseph G.; Warren, Stephen M.

In: Plastic and Reconstructive Surgery, Vol. 128, No. 2, 01.08.2011, p. 395-405.

Research output: Contribution to journalArticle

Wang, XX, Allen, RJ, Tutela, JP, Sailon, A, Allori, AC, Davidson, EH, Paek, GK, Saadeh, PB, McCarthy, JG & Warren, SM 2011, 'Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis', Plastic and Reconstructive Surgery, vol. 128, no. 2, pp. 395-405. https://doi.org/10.1097/PRS.0b013e31821e6e10
Wang, Xiao Xia ; Allen, Robert J. ; Tutela, John Paul ; Sailon, Alexander ; Allori, Alexander C. ; Davidson, Edward H. ; Paek, Gina K. ; Saadeh, Pierre B. ; McCarthy, Joseph G. ; Warren, Stephen M. / Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis. In: Plastic and Reconstructive Surgery. 2011 ; Vol. 128, No. 2. pp. 395-405.
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