Profile of volatile metabolites in urine by gas chromatography-mass spectrometry

Albert Zlatkis, Wolfgang Bertsch, H. A. Lichtenstein, Arye Tishbee, Farid Shunbo, M. Liebich, A. M. Coscia, N. Fleischer

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Profiles of volatile metabolites of 150 urine samples from normal individuals and 40 samples from subjects with diabetes mellitus have been studied by gas chromatography and mass spectrometry. The technique involves adsorption of the urinary headspace volatiles on Tenax GC, heat desorption with helium, trapping on a cooled precolumn, and chromatography on 100-m × 0.50-mm i.d. nickel columns. Individual profiles were observed over a period of 2 months. Characteristic constituents in normal urines are 2-butanone, 2-pentanone, 4-heptanone, dimethyl disulfide, several alkyl furans, pyrrole, and carvone. In subjects with diabetes mellitus under insulin treatment, high concentrations of pyrazines, cyclohexanone, lower aliphatic alcohols, and octanols were found.

Original languageEnglish (US)
Pages (from-to)763-767
Number of pages5
JournalAnalytical Chemistry
Volume45
Issue number4
StatePublished - 1973
Externally publishedYes

Fingerprint

Medical problems
Metabolites
Gas chromatography
Mass spectrometry
Furans
Pyrazines
Octanols
Helium
Pyrroles
Chromatography
Nickel
Desorption
Alcohols
Insulin
Adsorption
4-heptanone
carvone
2-pentanone
methylethyl ketone
Hot Temperature

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Zlatkis, A., Bertsch, W., Lichtenstein, H. A., Tishbee, A., Shunbo, F., Liebich, M., ... Fleischer, N. (1973). Profile of volatile metabolites in urine by gas chromatography-mass spectrometry. Analytical Chemistry, 45(4), 763-767.

Profile of volatile metabolites in urine by gas chromatography-mass spectrometry. / Zlatkis, Albert; Bertsch, Wolfgang; Lichtenstein, H. A.; Tishbee, Arye; Shunbo, Farid; Liebich, M.; Coscia, A. M.; Fleischer, N.

In: Analytical Chemistry, Vol. 45, No. 4, 1973, p. 763-767.

Research output: Contribution to journalArticle

Zlatkis, A, Bertsch, W, Lichtenstein, HA, Tishbee, A, Shunbo, F, Liebich, M, Coscia, AM & Fleischer, N 1973, 'Profile of volatile metabolites in urine by gas chromatography-mass spectrometry', Analytical Chemistry, vol. 45, no. 4, pp. 763-767.
Zlatkis A, Bertsch W, Lichtenstein HA, Tishbee A, Shunbo F, Liebich M et al. Profile of volatile metabolites in urine by gas chromatography-mass spectrometry. Analytical Chemistry. 1973;45(4):763-767.
Zlatkis, Albert ; Bertsch, Wolfgang ; Lichtenstein, H. A. ; Tishbee, Arye ; Shunbo, Farid ; Liebich, M. ; Coscia, A. M. ; Fleischer, N. / Profile of volatile metabolites in urine by gas chromatography-mass spectrometry. In: Analytical Chemistry. 1973 ; Vol. 45, No. 4. pp. 763-767.
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