Profile of Volatile Metabolites in Urine by Gas Chromatography-Mass Spectrometry

Albert Zlatkis; Wolfgang Bertsch; H. A. Lichtenstein; Arye Tishbee; Farid Shunbo; H. M. Liebich; A. M. Coscia; N. Fleischer

doi:10.1021/ac60326a036

Profile of Volatile Metabolites in Urine by Gas Chromatography-Mass Spectrometry

Albert Zlatkis, Wolfgang Bertsch, H. A. Lichtenstein, Arye Tishbee, Farid Shunbo, H. M. Liebich, A. M. Coscia, N. Fleischer

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

Profiles of volatile metabolites of 150 urine samples from normal individuals and 40 samples from subjects with diabetes mellitus have been studied by gas chromatography and mass spectrometry. The technique involves adsorption of the urinary headspace volatiles on Tenax GC, heat desorption with helium, trapping on a cooled pre-column, and chromatography on 100-m × 0.50-mm i.d. nickel columns. Individual profiles were observed over a period of 2 months. Characteristic constituents in normal urines are 2-butanone, 2-pentanone, 4-heptanone, dimethyl disulfide, several alkyl furans, pyrrole, and ca--vone. In subjects with diabetes mellitus under insulin treatment, high concentrations of pyrazines, cyclohexanone, lower aliphatic alcohols, and octanols were found.

Original language	English (US)
Pages (from-to)	763-767
Number of pages	5
Journal	Analytical Chemistry
Volume	45
Issue number	4
DOIs	https://doi.org/10.1021/ac60326a036
State	Published - Apr 1 1973
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac60326a036

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title = "Profile of Volatile Metabolites in Urine by Gas Chromatography-Mass Spectrometry",

abstract = "Profiles of volatile metabolites of 150 urine samples from normal individuals and 40 samples from subjects with diabetes mellitus have been studied by gas chromatography and mass spectrometry. The technique involves adsorption of the urinary headspace volatiles on Tenax GC, heat desorption with helium, trapping on a cooled pre-column, and chromatography on 100-m × 0.50-mm i.d. nickel columns. Individual profiles were observed over a period of 2 months. Characteristic constituents in normal urines are 2-butanone, 2-pentanone, 4-heptanone, dimethyl disulfide, several alkyl furans, pyrrole, and ca--vone. In subjects with diabetes mellitus under insulin treatment, high concentrations of pyrazines, cyclohexanone, lower aliphatic alcohols, and octanols were found.",

author = "Albert Zlatkis and Wolfgang Bertsch and Lichtenstein, {H. A.} and Arye Tishbee and Farid Shunbo and Liebich, {H. M.} and Coscia, {A. M.} and N. Fleischer",

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doi = "10.1021/ac60326a036",

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T1 - Profile of Volatile Metabolites in Urine by Gas Chromatography-Mass Spectrometry

AU - Zlatkis, Albert

AU - Bertsch, Wolfgang

AU - Lichtenstein, H. A.

AU - Tishbee, Arye

AU - Shunbo, Farid

AU - Liebich, H. M.

AU - Coscia, A. M.

AU - Fleischer, N.

PY - 1973/4/1

Y1 - 1973/4/1

N2 - Profiles of volatile metabolites of 150 urine samples from normal individuals and 40 samples from subjects with diabetes mellitus have been studied by gas chromatography and mass spectrometry. The technique involves adsorption of the urinary headspace volatiles on Tenax GC, heat desorption with helium, trapping on a cooled pre-column, and chromatography on 100-m × 0.50-mm i.d. nickel columns. Individual profiles were observed over a period of 2 months. Characteristic constituents in normal urines are 2-butanone, 2-pentanone, 4-heptanone, dimethyl disulfide, several alkyl furans, pyrrole, and ca--vone. In subjects with diabetes mellitus under insulin treatment, high concentrations of pyrazines, cyclohexanone, lower aliphatic alcohols, and octanols were found.

AB - Profiles of volatile metabolites of 150 urine samples from normal individuals and 40 samples from subjects with diabetes mellitus have been studied by gas chromatography and mass spectrometry. The technique involves adsorption of the urinary headspace volatiles on Tenax GC, heat desorption with helium, trapping on a cooled pre-column, and chromatography on 100-m × 0.50-mm i.d. nickel columns. Individual profiles were observed over a period of 2 months. Characteristic constituents in normal urines are 2-butanone, 2-pentanone, 4-heptanone, dimethyl disulfide, several alkyl furans, pyrrole, and ca--vone. In subjects with diabetes mellitus under insulin treatment, high concentrations of pyrazines, cyclohexanone, lower aliphatic alcohols, and octanols were found.

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Profile of Volatile Metabolites in Urine by Gas Chromatography-Mass Spectrometry

Abstract

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