Production of colony-stimulating factors (CFSs) during infection: Separate determinations of macrophage-, granulocyte-, granulocyte-macrophage-, and multi-CSFs

C. Cheers, A. M. Haigh, A. Kelso, D. Metcalf, E. R. Stanley, A. M. Young

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

After infection of mice with Listeria monocytogenes, elevated levels of colony-stimulating factors (CSFs) in the serum were quantitated by six different assays: ability to stimulate colony formation, the proliferation of 2 suspension of bone marrow cells (both measuring total colony-stimulating activity), a radioimmunoassay for macrophage-CSF (CSF-1), the WEHI-3B differentiation assay for granulocyte-CSF, and proliferation of 32D-c1-3 and FDC-P1 cell lines (specific for multi-CSF and either multi- or granulocyte-macrophage-CSFs, respectively). The great bulk of serum colony-stimulating activity represented macrophage- and granulocyte-CSFs, with small but measurable amounts of granulocyte-macrophage-CSF. The degree of elevation of serum CSF depended on the infecting dose used and the numbers of bacteria growing in the spleens and livers of the two mouse strains compared, i.e., L. monocytogenes-resistant C57BL/10 and susceptible BALB/cJ. The increase in serum CSFs occurred before the peak in bone marrow granulocyte-macrophage progenitors and before the reduction in bacterial numbers which follows the onset of specific cell-mediated immunity.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalInfection and Immunity
Volume56
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Colony-Stimulating Factors
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocytes
Macrophages
Macrophage Colony-Stimulating Factor
Listeria monocytogenes
Infection
Serum
Granulocyte-Macrophage Progenitor Cells
Granulocyte Colony-Stimulating Factor
Cellular Immunity
Bone Marrow Cells
Radioimmunoassay
Suspensions
Spleen
Bone Marrow
Bacteria
Cell Line
Liver

ASJC Scopus subject areas

  • Immunology

Cite this

Production of colony-stimulating factors (CFSs) during infection : Separate determinations of macrophage-, granulocyte-, granulocyte-macrophage-, and multi-CSFs. / Cheers, C.; Haigh, A. M.; Kelso, A.; Metcalf, D.; Stanley, E. R.; Young, A. M.

In: Infection and Immunity, Vol. 56, No. 1, 1988, p. 247-251.

Research output: Contribution to journalArticle

Cheers, C. ; Haigh, A. M. ; Kelso, A. ; Metcalf, D. ; Stanley, E. R. ; Young, A. M. / Production of colony-stimulating factors (CFSs) during infection : Separate determinations of macrophage-, granulocyte-, granulocyte-macrophage-, and multi-CSFs. In: Infection and Immunity. 1988 ; Vol. 56, No. 1. pp. 247-251.
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