TY - JOUR
T1 - Probing protein dynamics using temperature jump relaxation spectroscopy
AU - Callender, Robert
AU - Dyer, R. Brian
N1 - Funding Information:
This work was supported by the National Science Foundation, MCB-9727439 (RC), and the Institute of General Medicine, National Institutes of Health, GM53640 (RBD) and GM35183 (RC).
PY - 2002/10/1
Y1 - 2002/10/1
N2 - There have been recent advances in initiating and perturbing chemical reactions on very fast timescales, as short as picoseconds, thus making it feasible to study a vast range of chemical kinetics problems that heretofore could not be studied. One such approach is the rapid heating of water solutions using laser excitation. Laser-induced temperature jump relaxation spectroscopy can be used to determine the dynamics of protein motion, an area largely unstudied for want of suitable experimental and theoretical probes, despite the obvious importance of dynamics to protein function. Coupled with suitable spectroscopic probes of structure, relaxation spectroscopy can follow the motion of protein atoms over an enormous time range, from picoseconds to minutes (or longer), and with substantial structural specificity.
AB - There have been recent advances in initiating and perturbing chemical reactions on very fast timescales, as short as picoseconds, thus making it feasible to study a vast range of chemical kinetics problems that heretofore could not be studied. One such approach is the rapid heating of water solutions using laser excitation. Laser-induced temperature jump relaxation spectroscopy can be used to determine the dynamics of protein motion, an area largely unstudied for want of suitable experimental and theoretical probes, despite the obvious importance of dynamics to protein function. Coupled with suitable spectroscopic probes of structure, relaxation spectroscopy can follow the motion of protein atoms over an enormous time range, from picoseconds to minutes (or longer), and with substantial structural specificity.
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U2 - 10.1016/S0959-440X(02)00370-6
DO - 10.1016/S0959-440X(02)00370-6
M3 - Review article
C2 - 12464315
AN - SCOPUS:0036816569
SN - 0959-440X
VL - 12
SP - 628
EP - 633
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 5
ER -