Principles and approaches for reproducible scoring of tissue stains in research

David K. Meyerholz, Amanda P. Beck

Research output: Contribution to journalReview articlepeer-review

108 Scopus citations


Evaluation of tissues is a common and important aspect of translational research studies. Labeling techniques such as immunohistochemistry can stain cells/tissues to enhance identification of specific cell types, cellular activation states, and protein expression. While qualitative evaluation of labeled tissues has merit, use of semiquantitative and quantitative scoring approaches can greatly enhance the rigor of the tissue data. Adhering to key principles for reproducible scoring can enhance the quality and reproducibility of the tissue data so as to maximize its biological relevance and scientific impact.

Original languageEnglish (US)
Pages (from-to)844-855
Number of pages12
JournalLaboratory Investigation
Issue number7
StatePublished - Jul 1 2018

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology


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