Preparation of Fab fragments from a mouse monoclonal IgM

Jean M. Bidlack, Peter C. Mabie

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A procedure is described for preparing and purifying Fab fragments from a mouse monoclonal IgM. The IgM was digested with trypsin in the presence of reducing agent, cysteine. The resulting Fab fragments were alkylated with idoacetamide and purified using a Sephacryl S-200 column. The Fab fragments had a molecular weight of 48 000 as determined by SDS polyacrylamide gel electrophoresis and molecular sieve chromatography. This procedure has been successfully used with five different mouse monoclonal IgM. The Fab fragments bound antigen with the same specificity as the whole IgM.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalJournal of Immunological Methods
Volume91
Issue number2
DOIs
StatePublished - Jul 24 1986
Externally publishedYes

Fingerprint

Immunoglobulin Fab Fragments
Immunoglobulin M
Reducing Agents
Trypsin
Gel Chromatography
Cysteine
Polyacrylamide Gel Electrophoresis
Molecular Weight
Antigens

Keywords

  • Fab fragment
  • IgM, mouse
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Preparation of Fab fragments from a mouse monoclonal IgM. / Bidlack, Jean M.; Mabie, Peter C.

In: Journal of Immunological Methods, Vol. 91, No. 2, 24.07.1986, p. 157-162.

Research output: Contribution to journalArticle

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