Preparation and characterization of 15N2H3H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2

Walter Bodmer, Carl L. Schildkraut

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bacillus subtilis and T2 phage have been grown on a medium containing the following isotopically labeled compounds: 15N2H4Cl, thymidine-3H, deuterated sugars, and deuterated amino acids. The growth rates, titers, and thymidine-3H uptake are reported. 15N2H3H-DNA has been isolated from these two organisms. Some physical properties of these DNA samples are reported. Studies on genetic activity of the B. subtilis DNA do not reveal any change produced by the substitution of 15N2H3H for 14N1H.

Original languageEnglish (US)
Pages (from-to)229-243
Number of pages15
JournalAnalytical Biochemistry
Volume8
Issue number2
StatePublished - Jun 1964
Externally publishedYes

Fingerprint

Coliphages
Bacteriophage T4
Bacteriophages
Bacilli
Bacillus subtilis
Escherichia coli
Thymidine
DNA
Bacillus Phages
Sugars
Substitution reactions
Physical properties
Amino Acids
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Preparation and characterization of 15N2H3H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2. / Bodmer, Walter; Schildkraut, Carl L.

In: Analytical Biochemistry, Vol. 8, No. 2, 06.1964, p. 229-243.

Research output: Contribution to journalArticle

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