Preparation and characterization of 15N2H3H-labeled DNA from Bacillus subtilis and Escherichia coli phage T2

Walter Bodmer, Carl Schildkraut

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Bacillus subtilis and T2 phage have been grown on a medium containing the following isotopically labeled compounds: 15N2H4Cl, thymidine-3H, deuterated sugars, and deuterated amino acids. The growth rates, titers, and thymidine-3H uptake are reported. 15N2H3H-DNA has been isolated from these two organisms. Some physical properties of these DNA samples are reported. Studies on genetic activity of the B. subtilis DNA do not reveal any change produced by the substitution of 15N2H3H for 14N1H.

Original languageEnglish (US)
Pages (from-to)229-243
Number of pages15
JournalAnalytical Biochemistry
Volume8
Issue number2
DOIs
StatePublished - Jun 1964
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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