Prenatal morphine exposure alters ovarian steroid hormonal regulation of seizure susceptibility

Libor Velíšek, Jana Velíšková, Solomon L. Moshé, Ilona Vathy

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The present study examined the ovarian hormonal regulation of seizure susceptibility in prenatally morphine- and saline-exposed adult female rats in the flurothyl seizure model in vivo, and in low-magnesium-induced epileptiform activity in brain slices, in vitro. All females were ovariohysterectomized (OVX); some received either estrogen (E) or progesterone (P) replacement, while others were injected with E + P sequentially. In prenatally saline-treated control females, there was an increase in the flurothyl-induced clinic seizure threshold (anticonvulsant effect) in the presence of both hormones (E + P) compared to OVX controls. In morphine-exposed females, there was an increase in the flurothyl-induced clonic seizure threshold after an E injection alone while there was a reduced tonic-clonic seizure threshold in the presence of both hormones (E + P) compared to the hormone treatment-matched group of saline-exposed females. In control females, in low magnesium medium in vitro, the development of two types of epileptiform activity (seizure-like events and status of short discharges) was not affected by the different hormonal conditions. However, prenatal morphine exposure suppressed the development of both types of epileptiform activity in the E-injected females compared to the E-injected, control females. The present data demonstrate that the anticonvulsant effects of P on seizure susceptibility requires the presence of E. Furthermore, prenatal morphine exposure alters ovarian steroid hormone-regulated seizure susceptibility.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalBrain Research
Volume796
Issue number1-2
DOIs
StatePublished - Jun 15 1998

Keywords

  • Brainstem
  • Convulsion
  • Entorhinal cortex
  • Estrogen
  • Flurothyl
  • Forebrain
  • Low magnesium
  • Progesterone

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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