Preliminary results with sutured colonic anastomoses reinforced with dye-enhanced fibrinogen and a diode laser

Steven K. Libutti, Mathew R. Williams, Mehmet C. Oz, Kenneth A. Forde, Lawrence S. Bass, Samuel Weinstein, Joseph S. Auteri, Michael R. Treat, Roman Nowygrod

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A common cause of morbidity in patients recovering from bowel surgery is leakage from colonic anastomoses. We evaluated a technique utilizing a laser activated protein solder to strengthen colonic anastomoses in a canine model. Following creation of six single-layer interrupted suture anastomoses in four dogs, a protein solder consisting of indocyanine green dye and fibrinogen was topically applied to the serosal surface and exposed to 808 nm continuous wave diode laser energy. Immediately following anastomosis, the mean leakage pressure of sutures alone was 129 ± 14 mm hg (n=6), while the mean leakage pressure of sutures reinforced with the laser welded solder was 312 ± 32 mm hg (n=6) (p<0.001). Histologic examination of sections take through the anastomosis demonstrated a layer of fibrinogen across the anastomotic gap without evidence of thermal injury. Laser activated protein solder significantly enhances the immediate strength of sutured colonic anastomoses without causing appreciable thermal injury to surrounding tissues.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsGrahm M Watson, Rudolf W. Steiner, Joseph J. Pietrafitta
PublisherPubl by Int Soc for Optical Engineering
Pages169-172
Number of pages4
Volume1421
StatePublished - 1991
Externally publishedYes
EventProceedings of Lasers in Urology, Laparoscopy, and General Surgery - Los Angeles, CA, USA
Duration: Jan 21 1991Jan 23 1991

Other

OtherProceedings of Lasers in Urology, Laparoscopy, and General Surgery
CityLos Angeles, CA, USA
Period1/21/911/23/91

Fingerprint

fibrinogen
solders
Soldering alloys
Semiconductor lasers
Dyes
dyes
semiconductor lasers
leakage
proteins
Proteins
Lasers
lasers
dogs
surgery
Surgery
continuous radiation
examination
Tissue
causes
Hot Temperature

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Libutti, S. K., Williams, M. R., Oz, M. C., Forde, K. A., Bass, L. S., Weinstein, S., ... Nowygrod, R. (1991). Preliminary results with sutured colonic anastomoses reinforced with dye-enhanced fibrinogen and a diode laser. In G. M. Watson, R. W. Steiner, & J. J. Pietrafitta (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1421, pp. 169-172). Publ by Int Soc for Optical Engineering.

Preliminary results with sutured colonic anastomoses reinforced with dye-enhanced fibrinogen and a diode laser. / Libutti, Steven K.; Williams, Mathew R.; Oz, Mehmet C.; Forde, Kenneth A.; Bass, Lawrence S.; Weinstein, Samuel; Auteri, Joseph S.; Treat, Michael R.; Nowygrod, Roman.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Grahm M Watson; Rudolf W. Steiner; Joseph J. Pietrafitta. Vol. 1421 Publ by Int Soc for Optical Engineering, 1991. p. 169-172.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Libutti, SK, Williams, MR, Oz, MC, Forde, KA, Bass, LS, Weinstein, S, Auteri, JS, Treat, MR & Nowygrod, R 1991, Preliminary results with sutured colonic anastomoses reinforced with dye-enhanced fibrinogen and a diode laser. in GM Watson, RW Steiner & JJ Pietrafitta (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1421, Publ by Int Soc for Optical Engineering, pp. 169-172, Proceedings of Lasers in Urology, Laparoscopy, and General Surgery, Los Angeles, CA, USA, 1/21/91.
Libutti SK, Williams MR, Oz MC, Forde KA, Bass LS, Weinstein S et al. Preliminary results with sutured colonic anastomoses reinforced with dye-enhanced fibrinogen and a diode laser. In Watson GM, Steiner RW, Pietrafitta JJ, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1421. Publ by Int Soc for Optical Engineering. 1991. p. 169-172
Libutti, Steven K. ; Williams, Mathew R. ; Oz, Mehmet C. ; Forde, Kenneth A. ; Bass, Lawrence S. ; Weinstein, Samuel ; Auteri, Joseph S. ; Treat, Michael R. ; Nowygrod, Roman. / Preliminary results with sutured colonic anastomoses reinforced with dye-enhanced fibrinogen and a diode laser. Proceedings of SPIE - The International Society for Optical Engineering. editor / Grahm M Watson ; Rudolf W. Steiner ; Joseph J. Pietrafitta. Vol. 1421 Publ by Int Soc for Optical Engineering, 1991. pp. 169-172
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abstract = "A common cause of morbidity in patients recovering from bowel surgery is leakage from colonic anastomoses. We evaluated a technique utilizing a laser activated protein solder to strengthen colonic anastomoses in a canine model. Following creation of six single-layer interrupted suture anastomoses in four dogs, a protein solder consisting of indocyanine green dye and fibrinogen was topically applied to the serosal surface and exposed to 808 nm continuous wave diode laser energy. Immediately following anastomosis, the mean leakage pressure of sutures alone was 129 ± 14 mm hg (n=6), while the mean leakage pressure of sutures reinforced with the laser welded solder was 312 ± 32 mm hg (n=6) (p<0.001). Histologic examination of sections take through the anastomosis demonstrated a layer of fibrinogen across the anastomotic gap without evidence of thermal injury. Laser activated protein solder significantly enhances the immediate strength of sutured colonic anastomoses without causing appreciable thermal injury to surrounding tissues.",
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