Preexisting pancreatic acinar cells contribute to acinar cell, but not islet β cell, regeneration

Biva M. Desai, Jennifer M. Oliver-Krasinski, Diva D. De Leon, Cyrus Farzad, Nankang Hong, Steven D. Leach, Doris A. Stoffers

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

It has been suggested that pancreatic acinar cells can serve as progenitors for pancreatic islets, a concept with substantial implications for therapeutic efforts to increase insulin-producing β cell mass in patients with diabetes. We report what we believe to be the first in vivo lineage tracing approach to determine the plasticity potential of pancreatic acinar cells. We developed an acinar cell-specific inducible Cre recombinase transgenic mouse, which, when mated with a reporter strain and pulsed with tamoxifen, resulted in permanent and specific labeling of acinar cells and their progeny. During various time periods of observation and using several models to provoke injury, we failed to observe any chase of the labeled cells into the endocrine compartment, indicating that acinar cells do not normally transdifferentiate into islet β cells in vivo in adult mice. In contrast, we observed a substantial role for replication of preexisting acinar cells in the regeneration of new acinar cells after partial pancreatectomy. These results indicate that mature acinar cells harbor a facultative acinar but not endocrine progenitor capacity.

Original languageEnglish (US)
Pages (from-to)971-977
Number of pages7
JournalJournal of Clinical Investigation
Volume117
Issue number4
DOIs
StatePublished - Apr 2 2007
Externally publishedYes

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Acinar Cells
Islets of Langerhans
Regeneration
Pancreatectomy
Endocrine Cells
Tamoxifen
Transgenic Mice
Observation
Insulin
Wounds and Injuries

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Preexisting pancreatic acinar cells contribute to acinar cell, but not islet β cell, regeneration. / Desai, Biva M.; Oliver-Krasinski, Jennifer M.; De Leon, Diva D.; Farzad, Cyrus; Hong, Nankang; Leach, Steven D.; Stoffers, Doris A.

In: Journal of Clinical Investigation, Vol. 117, No. 4, 02.04.2007, p. 971-977.

Research output: Contribution to journalArticle

Desai, Biva M. ; Oliver-Krasinski, Jennifer M. ; De Leon, Diva D. ; Farzad, Cyrus ; Hong, Nankang ; Leach, Steven D. ; Stoffers, Doris A. / Preexisting pancreatic acinar cells contribute to acinar cell, but not islet β cell, regeneration. In: Journal of Clinical Investigation. 2007 ; Vol. 117, No. 4. pp. 971-977.
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