Abstract
Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from green turtles Chelonia mydas. These samples were taken from the carcasses of 10 juvenile green turtles with and without clinical signs of FP that stranded in Florida during 2014. Quantitative PCR for ChHV5 UL30 was used to identify ChHV5 DNA in tumors, skin, heart, kidney, nerves, and urine sampled from five out of five FP-positive and three out of five FP-free turtles. The most frequently co-occurring sites were cutaneous tumor and kidney (n = 4). Novel data presented here include the identification of ChHV5 DNA in kidney, heart, and nerve samples from three FP-free turtles. These data support candidate nontumored anatomic sites of ChHV5 DNA localization and mobilization during two different disease states that may be involved in the ChHV5 infection cycle. Received September 8, 2016; accepted April 17, 2017.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 136-142 |
| Number of pages | 7 |
| Journal | Journal of Aquatic Animal Health |
| Volume | 29 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2017 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Aquatic Science
Cite this
Potential Noncutaneous Sites of Chelonid Herpesvirus 5 Persistence and Shedding in Green Sea Turtles Chelonia mydas. / Page-Karjian, Annie; Gottdenker, Nicole L.; Whitfield, Jordyn; Herbst, Lawrence H.; Norton, Terry M.; Ritchie, Branson.
In: Journal of Aquatic Animal Health, Vol. 29, No. 3, 01.09.2017, p. 136-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Potential Noncutaneous Sites of Chelonid Herpesvirus 5 Persistence and Shedding in Green Sea Turtles Chelonia mydas
AU - Page-Karjian, Annie
AU - Gottdenker, Nicole L.
AU - Whitfield, Jordyn
AU - Herbst, Lawrence H.
AU - Norton, Terry M.
AU - Ritchie, Branson
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from green turtles Chelonia mydas. These samples were taken from the carcasses of 10 juvenile green turtles with and without clinical signs of FP that stranded in Florida during 2014. Quantitative PCR for ChHV5 UL30 was used to identify ChHV5 DNA in tumors, skin, heart, kidney, nerves, and urine sampled from five out of five FP-positive and three out of five FP-free turtles. The most frequently co-occurring sites were cutaneous tumor and kidney (n = 4). Novel data presented here include the identification of ChHV5 DNA in kidney, heart, and nerve samples from three FP-free turtles. These data support candidate nontumored anatomic sites of ChHV5 DNA localization and mobilization during two different disease states that may be involved in the ChHV5 infection cycle. Received September 8, 2016; accepted April 17, 2017.
AB - Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from green turtles Chelonia mydas. These samples were taken from the carcasses of 10 juvenile green turtles with and without clinical signs of FP that stranded in Florida during 2014. Quantitative PCR for ChHV5 UL30 was used to identify ChHV5 DNA in tumors, skin, heart, kidney, nerves, and urine sampled from five out of five FP-positive and three out of five FP-free turtles. The most frequently co-occurring sites were cutaneous tumor and kidney (n = 4). Novel data presented here include the identification of ChHV5 DNA in kidney, heart, and nerve samples from three FP-free turtles. These data support candidate nontumored anatomic sites of ChHV5 DNA localization and mobilization during two different disease states that may be involved in the ChHV5 infection cycle. Received September 8, 2016; accepted April 17, 2017.
UR - http://www.scopus.com/inward/record.url?scp=85046270468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046270468&partnerID=8YFLogxK
U2 - 10.1080/08997659.2017.1321590
DO - 10.1080/08997659.2017.1321590
M3 - Article
C2 - 28438079
AN - SCOPUS:85046270468
VL - 29
SP - 136
EP - 142
JO - Journal of Aquatic Animal Health
JF - Journal of Aquatic Animal Health
SN - 0899-7659
IS - 3
ER -