TY - JOUR
T1 - Potential Noncutaneous Sites of Chelonid Herpesvirus 5 Persistence and Shedding in Green Sea Turtles Chelonia mydas
AU - Page-Karjian, Annie
AU - Gottdenker, Nicole L.
AU - Whitfield, Jordyn
AU - Herbst, Lawrence
AU - Norton, Terry M.
AU - Ritchie, Branson
N1 - Funding Information:
This research was approved by the University of Georgia Office of the Vice President for Research Institutional Animal Care and Use Committee protocol A2012 10-011-Y1-A0. Samples from Florida turtles were collected under a scientific collecting permit (29-WJH-13-44) and a Special Purpose Importation permit (S2-WJH-13-2) from the Georgia Department of Natural Resources, Wildlife Resources Division, and a Marine Turtle Permit (No. 13-149) from the Florida Fish and Wildlife Conservation Commission. Financial and interdisciplinary support was provided by the Department of Pathology and the Infectious Diseases Laboratory of the University of Georgia, College of Veterinary Medicine. Funds were also provided by the American Association of Zoo Veterinarians’ Wild Animal Health Fund (2013-10). Corrie Brown and Mark Jackwood provided valuable critical feedback. The Sea Turtle Preservation Society, Volusia County Marine Science Center, Georgia Sea Turtle Center, and the Florida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute, provided sea turtle carcasses. Zhen Fu, Alvin Camus, and Monique Franca provided access to laboratory equipment. Meghan Koperski provided assistance with permits.
Publisher Copyright:
© American Fisheries Society 2017.
PY - 2017/9
Y1 - 2017/9
N2 - Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from green turtles Chelonia mydas. These samples were taken from the carcasses of 10 juvenile green turtles with and without clinical signs of FP that stranded in Florida during 2014. Quantitative PCR for ChHV5 UL30 was used to identify ChHV5 DNA in tumors, skin, heart, kidney, nerves, and urine sampled from five out of five FP-positive and three out of five FP-free turtles. The most frequently co-occurring sites were cutaneous tumor and kidney (n = 4). Novel data presented here include the identification of ChHV5 DNA in kidney, heart, and nerve samples from three FP-free turtles. These data support candidate nontumored anatomic sites of ChHV5 DNA localization and mobilization during two different disease states that may be involved in the ChHV5 infection cycle.
AB - Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from green turtles Chelonia mydas. These samples were taken from the carcasses of 10 juvenile green turtles with and without clinical signs of FP that stranded in Florida during 2014. Quantitative PCR for ChHV5 UL30 was used to identify ChHV5 DNA in tumors, skin, heart, kidney, nerves, and urine sampled from five out of five FP-positive and three out of five FP-free turtles. The most frequently co-occurring sites were cutaneous tumor and kidney (n = 4). Novel data presented here include the identification of ChHV5 DNA in kidney, heart, and nerve samples from three FP-free turtles. These data support candidate nontumored anatomic sites of ChHV5 DNA localization and mobilization during two different disease states that may be involved in the ChHV5 infection cycle.
UR - http://www.scopus.com/inward/record.url?scp=85046270468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046270468&partnerID=8YFLogxK
U2 - 10.1080/08997659.2017.1321590
DO - 10.1080/08997659.2017.1321590
M3 - Article
C2 - 28438079
AN - SCOPUS:85046270468
VL - 29
SP - 136
EP - 142
JO - Journal of Aquatic Animal Health
JF - Journal of Aquatic Animal Health
SN - 0899-7659
IS - 3
ER -