TY - JOUR
T1 - Polymorphisms of the IgH enhancer HS1.2 and risk of systemic lupus erythematosus
AU - Frezza, Domenico
AU - Tolusso, Barbara
AU - Giambra, Vincenzo
AU - Gremese, Elisa
AU - Marchini, Maurizio
AU - Nowik, Marcin
AU - Serone, Eliseo
AU - D'Addabbo, Pietro
AU - Mattioli, Claudia
AU - Canestri, Silvia
AU - Petricca, Luca
AU - D'Antona, Graziella
AU - Birshtein, Barbara K.
AU - Scorza, Raffaella
AU - Ferraccioli, Gianfranco
PY - 2012/8
Y1 - 2012/8
N2 - Objective: To determine whether the allelic frequency variation of the HS1.2 enhancer of the immunoglobulin heavy chain (IgH) 3′ regulatory region (3′RR-1) locus represents a risk factor for systemic lupus erythematosus (SLE) and to identify a possible functional difference in the two most frequent alleles (*1 and*2) in binding nuclear factor- κB (NF-κB) and Sp1. Methods: The frequency of the enhancer HS1.2 alleles was determined in two cohorts of patients with SLE (n=293) and in 1185 controls. Electrophoretic mobility shift assays (EMSA) were carried out with B cell nuclear extracts with different probes of HS1.2 alleles*1 and*2 to map the consensus binding sites of the nuclear factors. A confirmatory cohort of 121 patients with SLE was also included. Results: The frequency of allele*2 of the HS1.2 enhancer was significantly increased in patients with SLE compared with controls (OR 1.60, 95% CI 1.33 to 1.92, p<0.001). EMSA experiments showed the presence of the Sp1 binding site in both alleles whereas only allele*2 carried the consensus for the NF-κB factor. The presence versus absence of allele*2 in patients with SLE correlated with a higher concentration of IgM levels and with the expression of B cell activating factor receptor (BAFF-R). Conclusions: The increased frequency of allele*2 in patients with SLE identifies a new genetic risk factor for SLE. A possible biological effect of the polymorphism could be the difference observed in the localisation of an NF-κB binding site which is specific for allele*2 and absent in allele*1. These observations suggest a functional effect of the HS1.2 enhancer in this disease.
AB - Objective: To determine whether the allelic frequency variation of the HS1.2 enhancer of the immunoglobulin heavy chain (IgH) 3′ regulatory region (3′RR-1) locus represents a risk factor for systemic lupus erythematosus (SLE) and to identify a possible functional difference in the two most frequent alleles (*1 and*2) in binding nuclear factor- κB (NF-κB) and Sp1. Methods: The frequency of the enhancer HS1.2 alleles was determined in two cohorts of patients with SLE (n=293) and in 1185 controls. Electrophoretic mobility shift assays (EMSA) were carried out with B cell nuclear extracts with different probes of HS1.2 alleles*1 and*2 to map the consensus binding sites of the nuclear factors. A confirmatory cohort of 121 patients with SLE was also included. Results: The frequency of allele*2 of the HS1.2 enhancer was significantly increased in patients with SLE compared with controls (OR 1.60, 95% CI 1.33 to 1.92, p<0.001). EMSA experiments showed the presence of the Sp1 binding site in both alleles whereas only allele*2 carried the consensus for the NF-κB factor. The presence versus absence of allele*2 in patients with SLE correlated with a higher concentration of IgM levels and with the expression of B cell activating factor receptor (BAFF-R). Conclusions: The increased frequency of allele*2 in patients with SLE identifies a new genetic risk factor for SLE. A possible biological effect of the polymorphism could be the difference observed in the localisation of an NF-κB binding site which is specific for allele*2 and absent in allele*1. These observations suggest a functional effect of the HS1.2 enhancer in this disease.
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U2 - 10.1136/ard.2010.147025
DO - 10.1136/ard.2010.147025
M3 - Article
C2 - 22294636
AN - SCOPUS:84863840834
SN - 0003-4967
VL - 71
SP - 1309
EP - 1315
JO - Annals of the Rheumatic Diseases
JF - Annals of the Rheumatic Diseases
IS - 8
ER -