Point mutations cause the somatic diversification of IgM and IgG2a antiphosphorylcholine antibodies

N. C. Chien, R. R. Pollock, C. Desaymard, Matthew D. Scharff

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The genetic mechanism responsible for the somatic diversification of two mAbs was determined. The two PC-binding hybridomas were representative of events early and late in the immune response. The P28 cell line that produces an IgM antibody and thus represents events early in the immune response, was found to have 3 bp changes in its heavy chain variable (V(H)) region, with some changes in antibody affinity or specificity. The RP93 cell line that produces an IgG2a antibody and thus represents later events in the immune response, was found to have 9 bp changes in its V(H) region resulting in decreased affinity for PC and altered specificity. Oligonucleotides specific for linked base changes in the second hypervariable regions of both of these antibodies were used to look for previously undescribed V regions or other donor sequences that could have been responsible for these base changes. Since no donor sequences were found, we have concluded that somatic point mutation rather than gene conversion, V region replacement or the expression of an unidentified germline V(H) region gene is truly responsible for at least some of the somatic diversification of these antibodies.

Original languageEnglish (US)
Pages (from-to)954-973
Number of pages20
JournalJournal of Experimental Medicine
Volume167
Issue number3
StatePublished - 1988

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Point Mutation
Immunoglobulin M
Antibodies
Cell Line
Gene Conversion
Antibody Affinity
Antibody Specificity
Hybridomas
Oligonucleotides
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Point mutations cause the somatic diversification of IgM and IgG2a antiphosphorylcholine antibodies. / Chien, N. C.; Pollock, R. R.; Desaymard, C.; Scharff, Matthew D.

In: Journal of Experimental Medicine, Vol. 167, No. 3, 1988, p. 954-973.

Research output: Contribution to journalArticle

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