Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients but barely participate in interferon-α expression

Michelina Nascimbeni, Leïla Perié, Laurent Chorro, Seckou Diocou, Louis Kreitmann, Stéphanie Louis, Laurent Garderet, Bettina Fabiani, Anne Berger, Jürgen Schmitz, Jean Pierre Marie, Thierry Jo Molina, Jérôme Pacanowski, Jean Paul Viard, Eric Oksenhendler, Stéphanie Beq, Orly Abehsira-Amar, Rémi Cheynier, Anne Hosmalin

Research output: Contribution to journalArticle

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Abstract

We characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with high proviral loads. Moreover, although undetectable in most HIV- patients, interferon-α (IFN-α) production was evidenced in situ and by flow cytometry in most HIV+ patients. IFN-α was located in the marginal zone. Surprisingly, IFN-α colocalized only with few pDCs, but rather with other cells, including T and B lymphocytes, mDCs, and macrophages. Therefore, pDCs accumulated in spleens from HIV+ patients with high proviral loads, but they did not seem to be the main IFN-α producers.

Original languageEnglish (US)
Pages (from-to)6112-6119
Number of pages8
JournalBlood
Volume113
Issue number24
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Dendritic Cells
Interferons
Spleen
HIV
Myeloid Cells
Thrombocytopenic Purpura
Lymphocytes
Flow cytometry
Macrophages
Dextrans
Labeling
Pulp
Assays
Flow Cytometry
B-Lymphocytes
T-Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

Nascimbeni, M., Perié, L., Chorro, L., Diocou, S., Kreitmann, L., Louis, S., ... Hosmalin, A. (2009). Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients but barely participate in interferon-α expression. Blood, 113(24), 6112-6119. https://doi.org/10.1182/blood-2008-07-170803

Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients but barely participate in interferon-α expression. / Nascimbeni, Michelina; Perié, Leïla; Chorro, Laurent; Diocou, Seckou; Kreitmann, Louis; Louis, Stéphanie; Garderet, Laurent; Fabiani, Bettina; Berger, Anne; Schmitz, Jürgen; Marie, Jean Pierre; Molina, Thierry Jo; Pacanowski, Jérôme; Viard, Jean Paul; Oksenhendler, Eric; Beq, Stéphanie; Abehsira-Amar, Orly; Cheynier, Rémi; Hosmalin, Anne.

In: Blood, Vol. 113, No. 24, 2009, p. 6112-6119.

Research output: Contribution to journalArticle

Nascimbeni, M, Perié, L, Chorro, L, Diocou, S, Kreitmann, L, Louis, S, Garderet, L, Fabiani, B, Berger, A, Schmitz, J, Marie, JP, Molina, TJ, Pacanowski, J, Viard, JP, Oksenhendler, E, Beq, S, Abehsira-Amar, O, Cheynier, R & Hosmalin, A 2009, 'Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients but barely participate in interferon-α expression', Blood, vol. 113, no. 24, pp. 6112-6119. https://doi.org/10.1182/blood-2008-07-170803
Nascimbeni, Michelina ; Perié, Leïla ; Chorro, Laurent ; Diocou, Seckou ; Kreitmann, Louis ; Louis, Stéphanie ; Garderet, Laurent ; Fabiani, Bettina ; Berger, Anne ; Schmitz, Jürgen ; Marie, Jean Pierre ; Molina, Thierry Jo ; Pacanowski, Jérôme ; Viard, Jean Paul ; Oksenhendler, Eric ; Beq, Stéphanie ; Abehsira-Amar, Orly ; Cheynier, Rémi ; Hosmalin, Anne. / Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients but barely participate in interferon-α expression. In: Blood. 2009 ; Vol. 113, No. 24. pp. 6112-6119.
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