Placental 5-methylcytosine and 5-hydroxymethylcytosine patterns associate with size at birth

Chinthika Piyasena, Rebecca M. Reynolds, Khulan Batbayar, Jonathan R. Seckl, Gopi Menon, Amanda J. Drake

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Altered placental function as a consequence of aberrant imprinted gene expression may be one mechanism mediating the association between low birth weight and increased cardiometabolic disease risk. Imprinted gene expression is regulated by epigenetic mechanisms, particularly DNA methylation (5mC) at differentially methylated regions (DMRs). While 5-hydroxymethylcytosine (5hmC) is also present at DMRs, many techniques do not distinguish between 5mC and 5hmC. Using human placental samples, we show that the expression of the imprinted gene CDKN1C associates with birth weight. Using specific techniques to map 5mC and 5hmC at DMRs controlling the expression of CDKN1C and the imprinted gene IGF2, we show that 5mC enrichment at KvDMR and DMR0, and 5hmC enrichment within the H19 gene body, associate positively with birth weight. Importantly, the presence of 5hmC at imprinted DMRs may complicate the interpretation of DNA methylation studies in placenta; future studies should consider using techniques that distinguish between, and permit quantification of, both modifications.

Original languageEnglish (US)
Pages (from-to)692-697
Number of pages6
JournalEpigenetics
Volume10
Issue number8
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

5-Methylcytosine
Parturition
DNA Methylation
Gene Expression
Birth Weight
Low Birth Weight Infant
Epigenomics
Placenta
Genes
5-hydroxymethylcytosine

Keywords

  • 5-hydroxymethylcytosine
  • 5-methylcytosine
  • Birth weight
  • Imprinted gene
  • Placenta

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Placental 5-methylcytosine and 5-hydroxymethylcytosine patterns associate with size at birth. / Piyasena, Chinthika; Reynolds, Rebecca M.; Batbayar, Khulan; Seckl, Jonathan R.; Menon, Gopi; Drake, Amanda J.

In: Epigenetics, Vol. 10, No. 8, 01.01.2015, p. 692-697.

Research output: Contribution to journalArticle

Piyasena, Chinthika ; Reynolds, Rebecca M. ; Batbayar, Khulan ; Seckl, Jonathan R. ; Menon, Gopi ; Drake, Amanda J. / Placental 5-methylcytosine and 5-hydroxymethylcytosine patterns associate with size at birth. In: Epigenetics. 2015 ; Vol. 10, No. 8. pp. 692-697.
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