Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription

Dmitry Fyodorov, Tom Nelson, Evan Deneris

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We report a cDNA clone prepared from adrenal chromaffin-derived PC12 cell RNA that encodes a novel ETS-domain factor, Pet-1. The deduced primary structure of Pet-1 is composed of 340 amino acids and the encoded polypeptide has a predicted molecular mass of 35.4 kD. The pattern of Pet-1 gene expression in the neonatal rat is highly restricted and suggests that Pet-1 functions primarily in the nervous system. Adrenal gland expresses the highest level of Pet-1 among the tissues examined. In situ hybridization indicates that Pet-1 is expressed in the adrenal medulla but not the adrenal cortex. Slightly weaker Pet-1 hybridization is detected in brain and low levels are detectable in intestine and eye. Pet-1 can bind specifically to a PEA3 ETS DNA-binding motif and can modulate transcription of synthetic promoter constructs in a sequence-specific manner. We recently identified a neural cell-type specific enhancer, β43', within the 3'-untranslated exon of the neuronal nicotinic acetylcholine receptor (nAchR) β4 subunit gene. Similar to Pet-1, the β4 gene is also expressed in PC12 cells. The presence of putative ETS-domain binding sites in the β43' enhancer led us to hypothesize that members of the ets gene family activate neuronal nAchR genes. Cotransfection assays show that Pet-1 can activate reporter gene transcription in a β43' enhancer-dependent and cell type-dependent manner. Our results lead us to hypothesize that Pet-1 acts as a transcriptional regulator of downstream target genes involved in cholinergic neurotransmission.

Original languageEnglish (US)
Pages (from-to)151-163
Number of pages13
JournalJournal of Neurobiology
Volume34
Issue number2
DOIs
StatePublished - Feb 5 1998
Externally publishedYes

Fingerprint

Pets
Nicotinic Receptors
Genes
PC12 Cells
ETS Motif
Nucleotide Motifs
Adrenal Medulla
Adrenal Cortex
Adrenal Glands
Reporter Genes
Synaptic Transmission
Cholinergic Agents
Nervous System
Intestines
In Situ Hybridization
Exons
Complementary DNA
Clone Cells
Binding Sites
RNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription. / Fyodorov, Dmitry; Nelson, Tom; Deneris, Evan.

In: Journal of Neurobiology, Vol. 34, No. 2, 05.02.1998, p. 151-163.

Research output: Contribution to journalArticle

@article{3a42a514899d4d9680571d7208a953a0,
title = "Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription",
abstract = "We report a cDNA clone prepared from adrenal chromaffin-derived PC12 cell RNA that encodes a novel ETS-domain factor, Pet-1. The deduced primary structure of Pet-1 is composed of 340 amino acids and the encoded polypeptide has a predicted molecular mass of 35.4 kD. The pattern of Pet-1 gene expression in the neonatal rat is highly restricted and suggests that Pet-1 functions primarily in the nervous system. Adrenal gland expresses the highest level of Pet-1 among the tissues examined. In situ hybridization indicates that Pet-1 is expressed in the adrenal medulla but not the adrenal cortex. Slightly weaker Pet-1 hybridization is detected in brain and low levels are detectable in intestine and eye. Pet-1 can bind specifically to a PEA3 ETS DNA-binding motif and can modulate transcription of synthetic promoter constructs in a sequence-specific manner. We recently identified a neural cell-type specific enhancer, β43', within the 3'-untranslated exon of the neuronal nicotinic acetylcholine receptor (nAchR) β4 subunit gene. Similar to Pet-1, the β4 gene is also expressed in PC12 cells. The presence of putative ETS-domain binding sites in the β43' enhancer led us to hypothesize that members of the ets gene family activate neuronal nAchR genes. Cotransfection assays show that Pet-1 can activate reporter gene transcription in a β43' enhancer-dependent and cell type-dependent manner. Our results lead us to hypothesize that Pet-1 acts as a transcriptional regulator of downstream target genes involved in cholinergic neurotransmission.",
author = "Dmitry Fyodorov and Tom Nelson and Evan Deneris",
year = "1998",
month = "2",
day = "5",
doi = "10.1002/(SICI)1097-4695(19980205)34:2<151::AID-NEU5>3.0.CO;2-1",
language = "English (US)",
volume = "34",
pages = "151--163",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription

AU - Fyodorov, Dmitry

AU - Nelson, Tom

AU - Deneris, Evan

PY - 1998/2/5

Y1 - 1998/2/5

N2 - We report a cDNA clone prepared from adrenal chromaffin-derived PC12 cell RNA that encodes a novel ETS-domain factor, Pet-1. The deduced primary structure of Pet-1 is composed of 340 amino acids and the encoded polypeptide has a predicted molecular mass of 35.4 kD. The pattern of Pet-1 gene expression in the neonatal rat is highly restricted and suggests that Pet-1 functions primarily in the nervous system. Adrenal gland expresses the highest level of Pet-1 among the tissues examined. In situ hybridization indicates that Pet-1 is expressed in the adrenal medulla but not the adrenal cortex. Slightly weaker Pet-1 hybridization is detected in brain and low levels are detectable in intestine and eye. Pet-1 can bind specifically to a PEA3 ETS DNA-binding motif and can modulate transcription of synthetic promoter constructs in a sequence-specific manner. We recently identified a neural cell-type specific enhancer, β43', within the 3'-untranslated exon of the neuronal nicotinic acetylcholine receptor (nAchR) β4 subunit gene. Similar to Pet-1, the β4 gene is also expressed in PC12 cells. The presence of putative ETS-domain binding sites in the β43' enhancer led us to hypothesize that members of the ets gene family activate neuronal nAchR genes. Cotransfection assays show that Pet-1 can activate reporter gene transcription in a β43' enhancer-dependent and cell type-dependent manner. Our results lead us to hypothesize that Pet-1 acts as a transcriptional regulator of downstream target genes involved in cholinergic neurotransmission.

AB - We report a cDNA clone prepared from adrenal chromaffin-derived PC12 cell RNA that encodes a novel ETS-domain factor, Pet-1. The deduced primary structure of Pet-1 is composed of 340 amino acids and the encoded polypeptide has a predicted molecular mass of 35.4 kD. The pattern of Pet-1 gene expression in the neonatal rat is highly restricted and suggests that Pet-1 functions primarily in the nervous system. Adrenal gland expresses the highest level of Pet-1 among the tissues examined. In situ hybridization indicates that Pet-1 is expressed in the adrenal medulla but not the adrenal cortex. Slightly weaker Pet-1 hybridization is detected in brain and low levels are detectable in intestine and eye. Pet-1 can bind specifically to a PEA3 ETS DNA-binding motif and can modulate transcription of synthetic promoter constructs in a sequence-specific manner. We recently identified a neural cell-type specific enhancer, β43', within the 3'-untranslated exon of the neuronal nicotinic acetylcholine receptor (nAchR) β4 subunit gene. Similar to Pet-1, the β4 gene is also expressed in PC12 cells. The presence of putative ETS-domain binding sites in the β43' enhancer led us to hypothesize that members of the ets gene family activate neuronal nAchR genes. Cotransfection assays show that Pet-1 can activate reporter gene transcription in a β43' enhancer-dependent and cell type-dependent manner. Our results lead us to hypothesize that Pet-1 acts as a transcriptional regulator of downstream target genes involved in cholinergic neurotransmission.

UR - http://www.scopus.com/inward/record.url?scp=0031913451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031913451&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4695(19980205)34:2<151::AID-NEU5>3.0.CO;2-1

DO - 10.1002/(SICI)1097-4695(19980205)34:2<151::AID-NEU5>3.0.CO;2-1

M3 - Article

VL - 34

SP - 151

EP - 163

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 2

ER -