TY - JOUR
T1 - Permanent engraftment and function of hepatocytes delivered to the liver
T2 - Implications for gene therapy and Liver Repopulation
AU - Gupta, Sanjeev
AU - Aragona, Emma
AU - Vemuru, Ravikumar P.
AU - Bhargava, Kuldeep K.
AU - Burk, Robert D.
AU - Chowdhury, Jayanta Roy
PY - 1991/7
Y1 - 1991/7
N2 - To examine the distribution of intrasplenically transplanted hepatocytes, we used HBsAg‐producing G7 HBV transgenic hepatocytes or cells labeled with 111In. Most hepatocytes translocated to the liver (55% ± 7%; mean ± S.D.); the spleen retained a smaller fraction (15% ± 3%); and some transplanted cells localized in lungs (3%) or pancreas (1%). Transplanted hepatocytes were rapidly assimilated into the liver lobule. Morphometrical quantitation indicated that the numbers of transplanted hepatocytes in the liver at 48 hr and at 9 mo after transplantation were similar. Serum HBsAg was detected in recipients of the G7 HBV hepatocytes during the 1‐yr experiment. These results indicate that a large number of hepatocytes can be reproducibly delivered to the liver by transplantation into the spleen. Transplanted hepatocytes engraft rapidly, assimilate into host liver, maintain normal function and survive permanently. Systems for safe delivery and localization of hepatocytes in the liver represent a critical step toward successfully accomplishing hepatocyte‐directed gene therapy and repopulation of the acutely devastated liver. (HEPATOLOGY 1991;14:144–149.)
AB - To examine the distribution of intrasplenically transplanted hepatocytes, we used HBsAg‐producing G7 HBV transgenic hepatocytes or cells labeled with 111In. Most hepatocytes translocated to the liver (55% ± 7%; mean ± S.D.); the spleen retained a smaller fraction (15% ± 3%); and some transplanted cells localized in lungs (3%) or pancreas (1%). Transplanted hepatocytes were rapidly assimilated into the liver lobule. Morphometrical quantitation indicated that the numbers of transplanted hepatocytes in the liver at 48 hr and at 9 mo after transplantation were similar. Serum HBsAg was detected in recipients of the G7 HBV hepatocytes during the 1‐yr experiment. These results indicate that a large number of hepatocytes can be reproducibly delivered to the liver by transplantation into the spleen. Transplanted hepatocytes engraft rapidly, assimilate into host liver, maintain normal function and survive permanently. Systems for safe delivery and localization of hepatocytes in the liver represent a critical step toward successfully accomplishing hepatocyte‐directed gene therapy and repopulation of the acutely devastated liver. (HEPATOLOGY 1991;14:144–149.)
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U2 - 10.1002/hep.1840140124
DO - 10.1002/hep.1840140124
M3 - Article
C2 - 2066062
AN - SCOPUS:0025912865
SN - 0270-9139
VL - 14
SP - 144
EP - 149
JO - Hepatology
JF - Hepatology
IS - 1
ER -