Peptidomic analysis of human cell lines

Julia S. Gelman, Juan Sironi, Leandro M. Castro, Emer S. Ferro, Lloyd D. Fricker

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Peptides have been proposed to function in intracellular signaling within the cytosol. Although cytosolic peptides are considered to be highly unstable, a large number of peptides have been detected in mouse brain and other biological samples. In the present study, we evaluated the peptidome of three diverse cell lines: SH-SY5Y, MCF7, and HEK293 cells. A comparison of the peptidomes revealed considerable overlap in the identity of the peptides found in each cell line. The majority of the observed peptides are not derived from the most abundant or least stable proteins in the cell, and approximately half of the cellular peptides correspond to the N- or C- termini of the precursor proteins. Cleavage site analysis revealed a preference for hydrophobic residues in the P1 position. Quantitative peptidomic analysis indicated that the levels of most cellular peptides are not altered in response to elevated intracellular calcium, suggesting that calpain is not responsible for their production. The similarity of the peptidomes of the three cell lines and the lack of correlation with the predicted cellular degradome implies the selective formation or retention of these peptides, consistent with the hypothesis that they are functional in the cells.

Original languageEnglish (US)
Pages (from-to)1583-1592
Number of pages10
JournalJournal of Proteome Research
Volume10
Issue number4
DOIs
StatePublished - Apr 1 2011

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Cells
Cell Line
Peptides
Calpain
Protein Precursors
HEK293 Cells
MCF-7 Cells
Cytosol
Brain
Calcium
Chemical analysis
Proteins

Keywords

  • HEK293
  • hemopressin
  • MCF7
  • peptides
  • peptidomics
  • SH-SY5Y

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Gelman, J. S., Sironi, J., Castro, L. M., Ferro, E. S., & Fricker, L. D. (2011). Peptidomic analysis of human cell lines. Journal of Proteome Research, 10(4), 1583-1592. https://doi.org/10.1021/pr100952f

Peptidomic analysis of human cell lines. / Gelman, Julia S.; Sironi, Juan; Castro, Leandro M.; Ferro, Emer S.; Fricker, Lloyd D.

In: Journal of Proteome Research, Vol. 10, No. 4, 01.04.2011, p. 1583-1592.

Research output: Contribution to journalArticle

Gelman, JS, Sironi, J, Castro, LM, Ferro, ES & Fricker, LD 2011, 'Peptidomic analysis of human cell lines', Journal of Proteome Research, vol. 10, no. 4, pp. 1583-1592. https://doi.org/10.1021/pr100952f
Gelman, Julia S. ; Sironi, Juan ; Castro, Leandro M. ; Ferro, Emer S. ; Fricker, Lloyd D. / Peptidomic analysis of human cell lines. In: Journal of Proteome Research. 2011 ; Vol. 10, No. 4. pp. 1583-1592.
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