Pentacoordinate hemin derivatives in sodium dodecyl sulfate micelles: Model systems for the assignment of the fifth ligand in ferric heme proteins

Alberto Boffi, Tapan Kanti Das, Stefano Della Longa, Carla Spagnuolo, Denis L. Rousseau

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Ferric iron protoporhyrin IX derivatives in SDS micelles have been investigated by means of visible absorption, resonance Raman, and XANES spectroscopies to establish specific correlations between the marker bands of the pentacoordinate derivatives obtained from the three different techniques. Hydroxyl and 1,2-dimethyl imidazole coordinated hemins display the typical spectroscopic marker bands of a pentacoordinate high-spin ferric iron derivative in both Raman and XANES spectra. In turn, the optical absorption spectra of these two derivatives are very different. This difference is in line with the assignment of hydroxyl as the fifth coordination ligand to free hemin in SDS micelles, as demonstrated by the isotopic shift of the frequency of Fe-OH bond with H218O. The present assignments are relevant to the identification of the coordination state and the nature of the fifth ligand in ferric heme proteins.

Original languageEnglish (US)
Pages (from-to)1143-1149
Number of pages7
JournalBiophysical Journal
Volume77
Issue number2
StatePublished - Aug 1999

Fingerprint

Hemeproteins
Hemin
Micelles
Sodium Dodecyl Sulfate
Hydroxyl Radical
Iron
X-Ray Absorption Spectroscopy
Ligands
Raman Spectrum Analysis
imidazole

ASJC Scopus subject areas

  • Biophysics

Cite this

Pentacoordinate hemin derivatives in sodium dodecyl sulfate micelles : Model systems for the assignment of the fifth ligand in ferric heme proteins. / Boffi, Alberto; Das, Tapan Kanti; Della Longa, Stefano; Spagnuolo, Carla; Rousseau, Denis L.

In: Biophysical Journal, Vol. 77, No. 2, 08.1999, p. 1143-1149.

Research output: Contribution to journalArticle

@article{353a3d1a63944139aba66d0c17b7c3fc,
title = "Pentacoordinate hemin derivatives in sodium dodecyl sulfate micelles: Model systems for the assignment of the fifth ligand in ferric heme proteins",
abstract = "Ferric iron protoporhyrin IX derivatives in SDS micelles have been investigated by means of visible absorption, resonance Raman, and XANES spectroscopies to establish specific correlations between the marker bands of the pentacoordinate derivatives obtained from the three different techniques. Hydroxyl and 1,2-dimethyl imidazole coordinated hemins display the typical spectroscopic marker bands of a pentacoordinate high-spin ferric iron derivative in both Raman and XANES spectra. In turn, the optical absorption spectra of these two derivatives are very different. This difference is in line with the assignment of hydroxyl as the fifth coordination ligand to free hemin in SDS micelles, as demonstrated by the isotopic shift of the frequency of Fe-OH bond with H218O. The present assignments are relevant to the identification of the coordination state and the nature of the fifth ligand in ferric heme proteins.",
author = "Alberto Boffi and Das, {Tapan Kanti} and {Della Longa}, Stefano and Carla Spagnuolo and Rousseau, {Denis L.}",
year = "1999",
month = "8",
language = "English (US)",
volume = "77",
pages = "1143--1149",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "2",

}

TY - JOUR

T1 - Pentacoordinate hemin derivatives in sodium dodecyl sulfate micelles

T2 - Model systems for the assignment of the fifth ligand in ferric heme proteins

AU - Boffi, Alberto

AU - Das, Tapan Kanti

AU - Della Longa, Stefano

AU - Spagnuolo, Carla

AU - Rousseau, Denis L.

PY - 1999/8

Y1 - 1999/8

N2 - Ferric iron protoporhyrin IX derivatives in SDS micelles have been investigated by means of visible absorption, resonance Raman, and XANES spectroscopies to establish specific correlations between the marker bands of the pentacoordinate derivatives obtained from the three different techniques. Hydroxyl and 1,2-dimethyl imidazole coordinated hemins display the typical spectroscopic marker bands of a pentacoordinate high-spin ferric iron derivative in both Raman and XANES spectra. In turn, the optical absorption spectra of these two derivatives are very different. This difference is in line with the assignment of hydroxyl as the fifth coordination ligand to free hemin in SDS micelles, as demonstrated by the isotopic shift of the frequency of Fe-OH bond with H218O. The present assignments are relevant to the identification of the coordination state and the nature of the fifth ligand in ferric heme proteins.

AB - Ferric iron protoporhyrin IX derivatives in SDS micelles have been investigated by means of visible absorption, resonance Raman, and XANES spectroscopies to establish specific correlations between the marker bands of the pentacoordinate derivatives obtained from the three different techniques. Hydroxyl and 1,2-dimethyl imidazole coordinated hemins display the typical spectroscopic marker bands of a pentacoordinate high-spin ferric iron derivative in both Raman and XANES spectra. In turn, the optical absorption spectra of these two derivatives are very different. This difference is in line with the assignment of hydroxyl as the fifth coordination ligand to free hemin in SDS micelles, as demonstrated by the isotopic shift of the frequency of Fe-OH bond with H218O. The present assignments are relevant to the identification of the coordination state and the nature of the fifth ligand in ferric heme proteins.

UR - http://www.scopus.com/inward/record.url?scp=0032815529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032815529&partnerID=8YFLogxK

M3 - Article

C2 - 10423459

AN - SCOPUS:0032815529

VL - 77

SP - 1143

EP - 1149

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 2

ER -