PD-L1 is an activation-independent marker of brown adipocytes

Jessica R. Ingram, Michael Dougan, Mohammad Rashidian, Marko Knoll, Edmund J. Keliher, Sarah C. Garrett, Scott J. Garforth, Olga S. Blomberg, Camilo Espinosa, Atul Bhan, Steven C. Almo, Ralph Weissleder, Harvey Lodish, Stephanie K. Dougan, Hidde L. Ploegh

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.

Original languageEnglish (US)
Article number647
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Brown Adipocytes
death
markers
Chemical activation
activation
Ligands
ligands
Positron emission tomography
Brown Adipose Tissue
Positron-Emission Tomography
positrons
Single-Domain Antibodies
tomography
adrenergics
adipose tissues
fats
antibodies
Adrenergic Agents
Down-Regulation
cancer

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Ingram, J. R., Dougan, M., Rashidian, M., Knoll, M., Keliher, E. J., Garrett, S. C., ... Ploegh, H. L. (2017). PD-L1 is an activation-independent marker of brown adipocytes. Nature Communications, 8(1), [647]. https://doi.org/10.1038/s41467-017-00799-8

PD-L1 is an activation-independent marker of brown adipocytes. / Ingram, Jessica R.; Dougan, Michael; Rashidian, Mohammad; Knoll, Marko; Keliher, Edmund J.; Garrett, Sarah C.; Garforth, Scott J.; Blomberg, Olga S.; Espinosa, Camilo; Bhan, Atul; Almo, Steven C.; Weissleder, Ralph; Lodish, Harvey; Dougan, Stephanie K.; Ploegh, Hidde L.

In: Nature Communications, Vol. 8, No. 1, 647, 01.12.2017.

Research output: Contribution to journalArticle

Ingram, JR, Dougan, M, Rashidian, M, Knoll, M, Keliher, EJ, Garrett, SC, Garforth, SJ, Blomberg, OS, Espinosa, C, Bhan, A, Almo, SC, Weissleder, R, Lodish, H, Dougan, SK & Ploegh, HL 2017, 'PD-L1 is an activation-independent marker of brown adipocytes', Nature Communications, vol. 8, no. 1, 647. https://doi.org/10.1038/s41467-017-00799-8
Ingram, Jessica R. ; Dougan, Michael ; Rashidian, Mohammad ; Knoll, Marko ; Keliher, Edmund J. ; Garrett, Sarah C. ; Garforth, Scott J. ; Blomberg, Olga S. ; Espinosa, Camilo ; Bhan, Atul ; Almo, Steven C. ; Weissleder, Ralph ; Lodish, Harvey ; Dougan, Stephanie K. ; Ploegh, Hidde L. / PD-L1 is an activation-independent marker of brown adipocytes. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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