PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation

Yasuhito Tanaka, Ruth Pfeiffer, Anthony E T Yeo, Masashi Mizokami, Brian R. Edlin, Philip E. Castle, Thomas R. O'Brien, Harvey J. Alter, J. Wai Kuo Shih

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

SEN viruses (SENV) are newly discovered blood-borne single-stranded circular DNA viruses that may play a role in liver disease. To date, no serologic assays are available for the detection of SENV antigens or antibodies. We report on a rapid and sensitive molecular assay for the detection of four SENV strains (SENV-A, -C, -D, -H). This method uses PCR with universal primers and microwell capture hybridization with type-specific probes. Cut-off points to define "infected" based on chemiluminescence readings were determined from a statistical mixture model applied to samples from 300 injection drug users (IDUs) in San Francisco. Based on the estimated cut-off points, we examined the prevalence of SENV infection among 232 healthy US blood donors and assessed sensitivity and specificity of the assay in a small validation sample of infected individuals with partial sequence information.

Original languageEnglish (US)
Pages (from-to)123-130
Number of pages8
JournalJournal of Medical Virology
Volume73
Issue number1
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Torque teno virus
Statistical Models
Polymerase Chain Reaction
Circular DNA
DNA Viruses
San Francisco
Single-Stranded DNA
Virus Diseases
Luminescence
Drug Users
Blood Donors
Liver Diseases
Reading
Antigens
Sensitivity and Specificity
Injections
Antibodies

Keywords

  • Cut-off value
  • Injection drug users
  • Mixture model
  • Sensitivity
  • SENV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Tanaka, Y., Pfeiffer, R., Yeo, A. E. T., Mizokami, M., Edlin, B. R., Castle, P. E., ... Shih, J. W. K. (2004). PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation. Journal of Medical Virology, 73(1), 123-130. https://doi.org/10.1002/jmv.20067

PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation. / Tanaka, Yasuhito; Pfeiffer, Ruth; Yeo, Anthony E T; Mizokami, Masashi; Edlin, Brian R.; Castle, Philip E.; O'Brien, Thomas R.; Alter, Harvey J.; Shih, J. Wai Kuo.

In: Journal of Medical Virology, Vol. 73, No. 1, 05.2004, p. 123-130.

Research output: Contribution to journalArticle

Tanaka, Y, Pfeiffer, R, Yeo, AET, Mizokami, M, Edlin, BR, Castle, PE, O'Brien, TR, Alter, HJ & Shih, JWK 2004, 'PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation', Journal of Medical Virology, vol. 73, no. 1, pp. 123-130. https://doi.org/10.1002/jmv.20067
Tanaka, Yasuhito ; Pfeiffer, Ruth ; Yeo, Anthony E T ; Mizokami, Masashi ; Edlin, Brian R. ; Castle, Philip E. ; O'Brien, Thomas R. ; Alter, Harvey J. ; Shih, J. Wai Kuo. / PCR-Probe Capture Hybridization Assay and Statistical Model for SEN Virus Prevalence Estimation. In: Journal of Medical Virology. 2004 ; Vol. 73, No. 1. pp. 123-130.
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