Parsing redox potentials of five ferredoxins found within Thermotoga maritima

Stephanie J. Maiocco, Arthur J. Arcinas, Squire J. Booker, Sean J. Elliott

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Most organisms contain multiple soluble protein-based redox carriers such as members of the ferredoxin (Fd) family, that contain one or more iron–sulfur clusters. The potential redundancy of Fd proteins is poorly understood, particularly in connection to the ability of Fd proteins to deliver reducing equivalents to members of the “radical SAM,” or S-adenosylmethionine radical enzyme (ARE) superfamily, where the activity of all known AREs requires that an essential iron–sulfur cluster bound by the enzyme be reduced to the catalytically relevant [Fe4S4]1+ oxidation state. As it is still unclear whether a single Fd in a given organism is specific to individual redox partners, we have examined the five Fd proteins found within Thermotoga maritima via direct electrochemistry, to compare them in a side-by-side fashion for the first time. While a single [Fe4S4]-cluster bearing Fd (TM0927) has a potential of −420 mV, the other four 2x[Fe4S4]-bearing Fds (TM1175, TM1289, TM1533, and TM1815) have potentials that vary significantly, including cases where the two clusters of the same Fd are essentially coincident (e.g., TM1175) and those where the potentials are well separate (TM1815).

Original languageEnglish (US)
Pages (from-to)257-266
Number of pages10
JournalProtein Science
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Thermotoga maritima
Ferredoxins
Oxidation-Reduction
Bearings (structural)
Proteins
Electrochemistry
S-Adenosylmethionine
Aptitude
Enzymes
Redundancy
Oxidation

Keywords

  • ferredoxin
  • iron–sulfur cluster
  • redox homeostasis
  • redox potential
  • S-adenosylmethionine
  • thiomethylation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Parsing redox potentials of five ferredoxins found within Thermotoga maritima. / Maiocco, Stephanie J.; Arcinas, Arthur J.; Booker, Squire J.; Elliott, Sean J.

In: Protein Science, Vol. 28, No. 1, 01.01.2019, p. 257-266.

Research output: Contribution to journalArticle

Maiocco, Stephanie J. ; Arcinas, Arthur J. ; Booker, Squire J. ; Elliott, Sean J. / Parsing redox potentials of five ferredoxins found within Thermotoga maritima. In: Protein Science. 2019 ; Vol. 28, No. 1. pp. 257-266.
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